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Title: Production of aposporous gametophytes and calli from Pteris vittata L. pinnae strips cultured in vitro
Authors: Kwa, S.H.
Wee, Y.C. 
Loh, C.S. 
Keywords: aposporous gametophytes
in vitro culture
Pteris vittata
Issue Date: Oct-1991
Citation: Kwa, S.H.,Wee, Y.C.,Loh, C.S. (1991-10). Production of aposporous gametophytes and calli from Pteris vittata L. pinnae strips cultured in vitro. Plant Cell Reports 10 (8) : 392-393. ScholarBank@NUS Repository.
Abstract: Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata cultured in vitro at lower concentrations of all the mentioned components. Aposporous gametophytes and vegetative calli were produced at higher concentrations. The calli regenerated sporophytes when cultured on MS medium without growth regulators. The gametophytes grew vegetatively on MS medium but produced sporophytes when transferred into 0.1 strength MS medium. This is the first report of simultaneous production of calli and gametophytes from fern explants. © 1991 Springer-Verlag.
Source Title: Plant Cell Reports
ISSN: 07217714
DOI: 10.1007/BF00232608
Appears in Collections:Staff Publications

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