Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/99704
Title: Linamarin sensors: Amperometric sensing of linamarin using linamarase and glucose oxidase
Authors: Tatsuma, T.
Tani, K.
Oyama, N.
Yeoh, H.-H. 
Keywords: Amperometric sensors
Enzyme-modified electrodes
Linamarin
Issue Date: 10-May-1996
Source: Tatsuma, T.,Tani, K.,Oyama, N.,Yeoh, H.-H. (1996-05-10). Linamarin sensors: Amperometric sensing of linamarin using linamarase and glucose oxidase. Journal of Electroanalytical Chemistry 407 (1-2) : 155-159. ScholarBank@NUS Repository.
Abstract: Amperometric sensors for linamarin, a cyanogenic glycoside, were fabricated. They were based on the detection of glucose liberated from the hydrolysis of linamarin by linamarase from cassava leaf. A linamarase-glucose oxidase bienzyme-modified SnO2 electrode was observed to function at +900 mV vs. Ag | AgCl in a hydrogen peroxide detection mode, and could determine linamarin down to 10 μM. In contrast, a linamarase-glucose oxidase bienzyme-modified graphite electrode mediated by poly(N-isopropylacrylamide-co-vinylferrocene) responded to greater than 0.2 mM linamarin at +500 mV vs. Ag | AgCl. A linamarase-glucose oxidase-peroxidase trienzyme electrode was also fabricated by coating a peroxidase-incorporated polypyrrole-modified SnO2 electrode with a linamarase-glucose oxidase cross-linked film. This trienzyme electrode, which is the most practical linamarin sensor, was able to function at a mild potential of +150 mV vs. Ag | AgCl, and has a sensitivity of 5 μM linamarin.
Source Title: Journal of Electroanalytical Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/99704
ISSN: 00220728
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

25
checked on Feb 16, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.