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|Title:||Linamarin sensors: Amperometric sensing of linamarin using linamarase and glucose oxidase|
|Source:||Tatsuma, T.,Tani, K.,Oyama, N.,Yeoh, H.-H. (1996-05-10). Linamarin sensors: Amperometric sensing of linamarin using linamarase and glucose oxidase. Journal of Electroanalytical Chemistry 407 (1-2) : 155-159. ScholarBank@NUS Repository.|
|Abstract:||Amperometric sensors for linamarin, a cyanogenic glycoside, were fabricated. They were based on the detection of glucose liberated from the hydrolysis of linamarin by linamarase from cassava leaf. A linamarase-glucose oxidase bienzyme-modified SnO2 electrode was observed to function at +900 mV vs. Ag | AgCl in a hydrogen peroxide detection mode, and could determine linamarin down to 10 μM. In contrast, a linamarase-glucose oxidase bienzyme-modified graphite electrode mediated by poly(N-isopropylacrylamide-co-vinylferrocene) responded to greater than 0.2 mM linamarin at +500 mV vs. Ag | AgCl. A linamarase-glucose oxidase-peroxidase trienzyme electrode was also fabricated by coating a peroxidase-incorporated polypyrrole-modified SnO2 electrode with a linamarase-glucose oxidase cross-linked film. This trienzyme electrode, which is the most practical linamarin sensor, was able to function at a mild potential of +150 mV vs. Ag | AgCl, and has a sensitivity of 5 μM linamarin.|
|Source Title:||Journal of Electroanalytical Chemistry|
|Appears in Collections:||Staff Publications|
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