Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00232785
Title: An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture
Authors: Lakshmanan, P. 
Loh, C.-S. 
Goh, C.-J. 
Keywords: Aranda Deborah
orchids
thin section culture
tissue culture
Issue Date: May-1995
Source: Lakshmanan, P., Loh, C.-S., Goh, C.-J. (1995-05). An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture. Plant Cell Reports 14 (8) : 510-514. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00232785
Abstract: A thin section culture system for rapid regeneration of the monopodial orchid hybrid Aranda Deborah has been developed. Thin sections (0.6-0.7mm thick) obtained by transverse sectioning of a single shoot tip (6-7mm), when cultured in Vacin and Went medium enriched with coconut water (20% v/v), produced an average 13.6 protocorm-like bodies (PLB) after 45 days, compared to 2.7 PLB formed by a single 6-7 mm long shoot tip under same culture condition. Addition of α-naphthaleneacetic acid to Vacin and Went medium enriched with coconut water further increased PLB production by thin sections. PLB developed into plantlets on solid Vacin and Went medium containing 10% (v/v) coconut water and 0.5 g l-1 activated charcoal. With this procedure, more than 80,000 plantlets could be produced from thin sections obtained from a single shoot tip in a year as compared to nearly 11,000 plantlets produced by the conventional shoot tip method. © 1995 Springer-Verlag.
Source Title: Plant Cell Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/99649
ISSN: 07217714
DOI: 10.1007/BF00232785
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