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|Title:||An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture|
|Authors:||Lakshmanan, P. |
thin section culture
|Citation:||Lakshmanan, P., Loh, C.-S., Goh, C.-J. (1995-05). An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture. Plant Cell Reports 14 (8) : 510-514. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00232785|
|Abstract:||A thin section culture system for rapid regeneration of the monopodial orchid hybrid Aranda Deborah has been developed. Thin sections (0.6-0.7mm thick) obtained by transverse sectioning of a single shoot tip (6-7mm), when cultured in Vacin and Went medium enriched with coconut water (20% v/v), produced an average 13.6 protocorm-like bodies (PLB) after 45 days, compared to 2.7 PLB formed by a single 6-7 mm long shoot tip under same culture condition. Addition of α-naphthaleneacetic acid to Vacin and Went medium enriched with coconut water further increased PLB production by thin sections. PLB developed into plantlets on solid Vacin and Went medium containing 10% (v/v) coconut water and 0.5 g l-1 activated charcoal. With this procedure, more than 80,000 plantlets could be produced from thin sections obtained from a single shoot tip in a year as compared to nearly 11,000 plantlets produced by the conventional shoot tip method. © 1995 Springer-Verlag.|
|Source Title:||Plant Cell Reports|
|Appears in Collections:||Staff Publications|
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