Please use this identifier to cite or link to this item: https://doi.org/10.1006/bbrc.1995.2504
Title: A single base-pair change (ATG → ATC) nullifies the activity of cytosolic fumarase in Saccharomyces cerevisiae
Authors: Wu, M.
Wong, S.-M. 
Tan, H.-M.
Ting, R.
Issue Date: 1995
Source: Wu, M., Wong, S.-M., Tan, H.-M., Ting, R. (1995). A single base-pair change (ATG → ATC) nullifies the activity of cytosolic fumarase in Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 215 (2) : 578-590. ScholarBank@NUS Repository. https://doi.org/10.1006/bbrc.1995.2504
Abstract: A respiratory defective pet mutant (W303VFUM1) of Saccharomyces cerevisiae deficient in fumarase was transformed with the plasmid construct pG5/ST7. This plasmid contains the entire FUM1 gene with only a single base pair change (ATG → ATC) confined to the putative second inframe translation initiation codon. While transformation of the fumarase deficient mutant with pG5/ST7 resulted in an elevation of fumarase activity in the mitochondria of the transformed strain, fumarase activity in the cytosol remained negligible. This result indicated that the cytosolic fumarase isoenzyme is synthesized exclusively from the second translation initiation codon of FUM1. Results of RACE-PCR of the 5' ends of FUM1 transcripts confirmed that the FUM1 gene synthesizes two distinct species of transcripts. These data provide strong evidence for the synthesis and targeting of two fumarase isomers.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/99637
ISSN: 0006291X
DOI: 10.1006/bbrc.1995.2504
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

2
checked on Jan 23, 2018

WEB OF SCIENCETM
Citations

3
checked on Jan 10, 2018

Page view(s)

42
checked on Jan 22, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.