Please use this identifier to cite or link to this item:
|Title:||Nuclear microscopy: A novel technique for quantitative imaging of gadolinium Distribution within Tissue Sections|
|Authors:||Rajendran, R. |
Magnetic resonance imaging
|Source:||Rajendran, R., Ronald, J.A., Ye, T., Minqin, R., Chen, J.W., Weissleder, R., Rutt, B.K., Halliwell, B., Watt, F. (2009-08). Nuclear microscopy: A novel technique for quantitative imaging of gadolinium Distribution within Tissue Sections. Microscopy and Microanalysis 15 (4) : 338-344. ScholarBank@NUS Repository. https://doi.org/10.1017/S1431927609090813|
|Abstract:||All clinically-approved and many novel gadolinium (Gd)-based contrast agents used to enhance signal intensity in magnetic resonance imaging (MRI) are optically silent. To verify MRI results, a gold standard that can map and quantify Gd down to the parts per million (ppm) levels is required. Nuclear microscopy is a relatively new technique that has this capability and is composed of a combination of three ion beam techniques: scanning transmission ion microscopy, Rutherford backscattering spectrometry, and particle induced X-ray emission used in conjunction with a high energy proton microprobe. In this proof-of-concept study, we show that in diseased aortic vessel walls obtained at 2 and 4 h after intravenous injection of the myeloperoxidase-senstitive MRI agent, bis-5-hydroxytryptamide-diethylenetriamine-pentaacetate gadolinium, there was a time-dependant Gd clearance (2 h = 18.86 ppm, 4 h = 8.65 ppm). As expected, the control animal, injected with the clinically-approved conventional agent diethylenetriamine-pentaacetate gadolinium and sacrificed 1 week after injection, revealed no significant residual Gd in the tissue. Similar to known in vivo Gd pharmacokinetics, we found that Gd concentration dropped by a factor of 2 in vessel wall tissue in 1.64 h. Further high-resolution studies revealed that Gd was relatively uniformly distributed, consistent with random agent diffusion. We conclude that nuclear microscopy is potentially very useful for validation studies involving Gd-based magnetic resonance contrast agents. © Microscopy Society of America 2009.|
|Source Title:||Microscopy and Microanalysis|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 27, 2018
WEB OF SCIENCETM
checked on Feb 27, 2018
checked on Feb 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.