Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.nimb.2012.11.047
Title: Whole cell structural imaging at 20 nanometre resolutions using MeV ions
Authors: Watt, F. 
Chen, X.
Chen, C.-B. 
Udalagama, C.N.B. 
Van Kan, J.A. 
Bettiol, A.A. 
Keywords: Gold nanoparticles
Scanning transmission ion microscopy
Whole cell imaging
Issue Date: 2013
Citation: Watt, F., Chen, X., Chen, C.-B., Udalagama, C.N.B., Van Kan, J.A., Bettiol, A.A. (2013). Whole cell structural imaging at 20 nanometre resolutions using MeV ions. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms 306 : 6-11. ScholarBank@NUS Repository. https://doi.org/10.1016/j.nimb.2012.11.047
Abstract: MeV proton and alpha (helium ion) particle beams can now be focused to 20 nm spot sizes, and ion/matter simulations using the DEEP computer code show that these resolutions are maintained through the top micrometre or so of organic material. In addition, the energy deposition profiles of the transmitted ions are laterally constrained to a few nanometers from the initial ion path. This paves the way for high resolution structural imaging of relatively thick biological material, e.g. biological cells. Examples are shown of high resolution structural imaging of whole biological cells (MRC5) using on-axis scanning transmission ion microscopy (STIM). Nanoparticles have the ability to cross the cell membrane, and may therefore prove useful as drug delivery probes. We show that the combination of on-axis STIM for imaging the cell interior, and off-axis STIM for imaging gold nanoparticles with enhanced contrast within the cell, represents a powerful set of ion beam techniques for tracking gold nanoparticles in biological cells. Whole cell imaging at high spatial resolutions represents a new area for nuclear microprobes. © 2013 Elsevier B.V. All rights reserved.
Source Title: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
URI: http://scholarbank.nus.edu.sg/handle/10635/98584
ISSN: 0168583X
DOI: 10.1016/j.nimb.2012.11.047
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