Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0168-583X(98)00903-3
Title: Single cell elemental analysis using nuclear microscopy
Authors: Ren, M.Q. 
Thong, P.S.P. 
Kara, U. 
Watt, F. 
Issue Date: 2-Apr-1999
Citation: Ren, M.Q., Thong, P.S.P., Kara, U., Watt, F. (1999-04-02). Single cell elemental analysis using nuclear microscopy. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms 150 (1-4) : 179-184. ScholarBank@NUS Repository. https://doi.org/10.1016/S0168-583X(98)00903-3
Abstract: The use of Particle Induced X-ray Emission (PIXE), Rutherford Backscattering Spectrometry (RBS) and Scanning Transmission Ion Microscopy (STIM) to provide quantitative elemental analysis of single cells in an area which has high potential, particularly when the trace elements such as Ca, Fe, Zn and Cu can be monitored. We describe the methodology of sample preparation for two cell types, the procedures of cell imaging using STIM, and the quantitative elemental analysis of single cells using RBS and PIXE. Recent work on single cells at the Nuclear Microscopy Research Centre, National University of Singapore has centred around two research areas: (a) Apoptosis (programmed cell death), which has been recently implicated in a wide range of pathological conditions such as cancer, Parkinson's disease etc, and (b) Malaria (infection of red blood cells by the malaria parasite). Firstly we present results on the elemental analysis of human Chang liver cells (ATTCC CCL 13) where vanadium ions were used to trigger apoptosis, and demonstrate that nuclear microscopy has the capability of monitoring vanadium loading within individual cells. Secondly we present the results of elemental changes taking place in individual mouse red blood cells which have been infected with the malaria parasite and treated with the anti-malaria drug Qinghaosu (QHS).
Source Title: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
URI: http://scholarbank.nus.edu.sg/handle/10635/97935
ISSN: 0168583X
DOI: 10.1016/S0168-583X(98)00903-3
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

8
checked on Oct 19, 2018

WEB OF SCIENCETM
Citations

7
checked on Oct 3, 2018

Page view(s)

40
checked on Sep 28, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.