Please use this identifier to cite or link to this item: https://doi.org/10.1366/000370202760295340
Title: Raman microspectroscopy of normal erythrocytes and plasmodium berghei-infected erythrocytes
Authors: Ong, C.W.
Shen, Z.X. 
Ang, K.K.H.
Kara, U.A.K. 
Tang, S.H. 
Keywords: β-haematin
Ferri-protoporphyrin
Haemozoin
Malaria
Raman spectroscopy
Issue Date: Sep-2002
Citation: Ong, C.W., Shen, Z.X., Ang, K.K.H., Kara, U.A.K., Tang, S.H. (2002-09). Raman microspectroscopy of normal erythrocytes and plasmodium berghei-infected erythrocytes. Applied Spectroscopy 56 (9) : 1126-1131. ScholarBank@NUS Repository. https://doi.org/10.1366/000370202760295340
Abstract: Raman spectroscopy was applied to study β-haematin, normal erythrocytes, parasite-infected erythrocytes, and lysed samples of the parasite-infected erythrocytes. Differences were observed between spectra excited by the Ar+ laser and those excited by the HeNe laser. The significant difference lies in the fact that with the HeNe laser excitation at 632.8 nm, band absorption is a result of the charge transfer, while laser excitation in Qv-absorption bands of the active site of the haem1 occurs for the Ar+ laser. Similarity in the spectra of β-haematin and parasite-infected erythrocyte was observed with the HeNe laser. The differences between the normal erythrocyte and the parasite-infected erythrocyte may still be distinguished by the band at 754 cm-1 for the parasite-infected erythrocyte. In addition, with the HeNe laser, the band at 1080 cm-1 may also be used as a reference to whether the erythrocyte has been infected.
Source Title: Applied Spectroscopy
URI: http://scholarbank.nus.edu.sg/handle/10635/97732
ISSN: 00037028
DOI: 10.1366/000370202760295340
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