Please use this identifier to cite or link to this item:
Title: Nanouidic compaction of DNA by like-charged protein
Authors: Zhang, C.
Gong, Z.
Guttula, D.
Malar, P.P. 
Van Kan, J.A. 
Doyle, P.S.
Van Der Maarel, J.R.C. 
Issue Date: 8-Mar-2012
Source: Zhang, C., Gong, Z., Guttula, D., Malar, P.P., Van Kan, J.A., Doyle, P.S., Van Der Maarel, J.R.C. (2012-03-08). Nanouidic compaction of DNA by like-charged protein. Journal of Physical Chemistry B 116 (9) : 3031-3036. ScholarBank@NUS Repository.
Abstract: The effects of the like-charged proteins bovine serum albumin and hemoglobin on the conformation and compaction of single DNA molecules confined in rectangular nanochannels were investigated with fluorescence microscopy. The channels have lengths of 50 μm and cross-sectional diameters in the range of 80-300 nm. In the wider channels, the DNA molecules are compressed and eventually condense into a compact form with increasing concentration of protein. In the narrow channels, no condensation was observed. The threshold concentration for condensation depends on the channel cross-sectional diameter as well as the ionic strength of the supporting medium. The critical values for full compaction are typically less than one-tenth of a millimolar. In the bulk phase and in the same environmental conditions, no condensation was observed. Anisotropic nanoconfinement hence facilitates compaction of DNA by negatively charged protein. We tentatively interpret this behavior in terms of enhanced depletion interaction between segments of the DNA molecule due to orientation order imposed by the channel walls. © 2012 American Chemical Society.
Source Title: Journal of Physical Chemistry B
ISSN: 15206106
DOI: 10.1021/jp2124907
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Feb 27, 2018


checked on Feb 14, 2018

Page view(s)

checked on Mar 12, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.