Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.nimb.2012.12.052
Title: Ion-induced fluorescence imaging of endosomes
Authors: Norarat, R.
Marjomäki, V.
Chen, X.
Zhaohong, M.
Minqin, R. 
Chen, C.-B. 
Bettiol, A.A. 
Whitlow, H.J.
Watt, F. 
Keywords: Autofluorescence
Diffraction limit
Endosomes
Proton-induced fluorescence (PIF)
Scanning Transmission Ion Microscopy (STIM)
Issue Date: 2013
Citation: Norarat, R., Marjomäki, V., Chen, X., Zhaohong, M., Minqin, R., Chen, C.-B., Bettiol, A.A., Whitlow, H.J., Watt, F. (2013). Ion-induced fluorescence imaging of endosomes. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms 306 : 113-116. ScholarBank@NUS Repository. https://doi.org/10.1016/j.nimb.2012.12.052
Abstract: Imaging laboratories at Jyväskylä and Singapore are collaborating on the development of fluorescence imaging of cytoplasmic endosomes using a combination of proton induced fluorescence (PIF) with direct Scanning Transmission Ion Microscopy (direct-STIM) for sub-cellular structural imaging. A549 lung carcinoma cells were cultivated and stained for epidermal growth factor receptor (EGFR) and receptor α2β1 integrin. In this paper, we demonstrate that cells can be imaged at sub-150 nm resolution using the PIF technique. In addition, the same target cell was imaged at 50 and 25 nm resolution by using proton and He-STIM, respectively. The combination of both techniques offer a powerful tool to improve fluorescence imaging beyond optical diffraction limits. © 2013 Elsevier B.V. All rights reserved.
Source Title: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
URI: http://scholarbank.nus.edu.sg/handle/10635/96998
ISSN: 0168583X
DOI: 10.1016/j.nimb.2012.12.052
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