Please use this identifier to cite or link to this item:
|Title:||Injectable biodegradable polyethylene glycol/ RGD peptide hybrid hydrogels for in vitro chondrogenesis of human mesenchymal stern cellsa|
|Authors:||Qiong Liu, S. |
Po Hui, J.H.
Yan Yang, Y.
Human mesenchymal stem cells
Injectable peg hydrogel
|Citation:||Qiong Liu, S., Tian, Q., Wang, L., Hedrick, J.L., Po Hui, J.H., Yan Yang, Y., Ee, P.L.R. (2010-07-01). Injectable biodegradable polyethylene glycol/ RGD peptide hybrid hydrogels for in vitro chondrogenesis of human mesenchymal stern cellsa. Macromolecular Rapid Communications 31 (13) : 1148-1154. ScholarBank@NUS Repository. https://doi.org/10.1002/marc.200900818|
|Abstract:||(Figure Presented) In this study, an injectable and biodegradable poly(ethylene glycol) (PEG)/arginine-glycine-aspartic (RGD) peptide hybrid hydrogel has been synthesized and used as a biomimetic scaffold for encapsulation of human mesenchymal stem cells (hMSCs). Tetrahydroxyl PEG was functionalized with acrylate, and then reacted with thiol-containing peptide (RGD). Gelation occurred within 30 min with the addition of cells and PEG-dithiol via Michael addition. The hydrogels synthesized with a peptide concentration of 1.0-5.0 mM achieved significantly greater cell viability when compared to the hydrogels without the RGD peptide. However, the effect of RGD on chondrogenesis was found to be dose-dependent. Immunohistology studies demonstrated that hMSCs encapsulated in the hydrogel matrix with 1.0 mM RGD and TGF-ß3 showed enhanced positive staining for aggrecan and type II collagen as compared to that with 5.0 mM RGD and unmodified PEG hydrogels. RT-PCR results further revealed that the cells in hydrogels with 1 mM RGD expressed significantly higher levels of type II collagen than those in PEG hydogels without RGD peptide. These findings have demonstrated that the PEG-RGD hydrogels can be a promising scaffold to deliver hMSCs for cartilage repair. © 2010 WILEY-VCH Verlag GmbH & Co. KCaA, Weinheim.|
|Source Title:||Macromolecular Rapid Communications|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 20, 2019
WEB OF SCIENCETM
checked on Mar 20, 2019
checked on Jan 25, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.