Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ajpath.2011.03.013
Title: Excessive neutrophils and neutrophil extracellular traps contribute to acute lung injury of influenza pneumonitis
Authors: Narasaraju, T.
Yang, E.
Samy, R.P. 
Ng, H.H.
Poh, W.P.
Liew, A.-A.
Phoon, M.C.
Van Rooijen, N.
Chow, V.T.
Issue Date: Jul-2011
Citation: Narasaraju, T., Yang, E., Samy, R.P., Ng, H.H., Poh, W.P., Liew, A.-A., Phoon, M.C., Van Rooijen, N., Chow, V.T. (2011-07). Excessive neutrophils and neutrophil extracellular traps contribute to acute lung injury of influenza pneumonitis. American Journal of Pathology 179 (1) : 199-210. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ajpath.2011.03.013
Abstract: Complications of acute respiratory distress syndrome (ARDS) are common among critically ill patients infected with highly pathogenic influenza viruses. Macrophages and neutrophils constitute the majority of cells recruited into infected lungs, and are associated with immunopathology in influenza pneumonia. We examined pathological manifestations in models of macrophage- or neutrophil-depleted mice challenged with sublethal doses of influenza A virus H1N1 strain PR8. Infected mice depleted of macrophages displayed excessive neutrophilic infiltration, alveolar damage, and increased viral load, later progressing into ARDS-like pathological signs with diffuse alveolar damage, pulmonary edema, hemorrhage, and hypoxemia. In contrast, neutrophil-depleted animals showed mild pathology in lungs. The brochoalveolar lavage fluid of infected macrophage-depleted mice exhibited elevated protein content, T1-α, thrombomodulin, matrix metalloproteinase-9, and myeloperoxidase activities indicating augmented alveolar-capillary damage, compared to neutrophil-depleted animals. We provide evidence for the formation of neutrophil extracellular traps (NETs), entangled with alveoli in areas of tissue injury, suggesting their potential link with lung damage. When co-incubated with infected alveolar epithelial cells in vitro, neutrophils from infected lungs strongly induced NETs generation, and augmented endothelial damage. NETs induction was abrogated by anti-myeloperoxidase antibody and an inhibitor of superoxide dismutase, thus implying that NETs generation is induced by redox enzymes in influenza pneumonia. These findings support the pathogenic effects of excessive neutrophils in acute lung injury of influenza pneumonia by instigating alveolar-capillary damage. © 2011 American Society for Investigative Pathology.
Source Title: American Journal of Pathology
URI: http://scholarbank.nus.edu.sg/handle/10635/96542
ISSN: 00029440
DOI: 10.1016/j.ajpath.2011.03.013
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

271
checked on Nov 21, 2018

WEB OF SCIENCETM
Citations

258
checked on Nov 21, 2018

Page view(s)

33
checked on Nov 9, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.