Please use this identifier to cite or link to this item: https://doi.org/10.1046/j.1471-4159.1999.721574.x
Title: A nuclear microscopic study of elemental changes in the rat hippocampus after kainate-induced neuronal injury
Authors: Ong, W.-Y.
Ren, M.-Q. 
Makjanić, J. 
Lim, T.-M. 
Watt, F. 
Keywords: Alzheimer's disease
Astrocytes
Calcium
Excitotoxicity
Macrophages
Nuclear microscope
Issue Date: 1999
Citation: Ong, W.-Y., Ren, M.-Q., Makjanić, J., Lim, T.-M., Watt, F. (1999). A nuclear microscopic study of elemental changes in the rat hippocampus after kainate-induced neuronal injury. Journal of Neurochemistry 72 (4) : 1574-1579. ScholarBank@NUS Repository. https://doi.org/10.1046/j.1471-4159.1999.721574.x
Abstract: The effect of intracerebroventricular kainate injection on the elemental composition of the hippocampus was studied in adult Wistar rats, at 1 day and 1, 2, 3, and 4 weeks postinjection, using a nuclear microscope. An increase in calcium concentration was observed on the injected side from 1 day postinjection. The increase peaked at 3 weeks postinjection, reaching a concentration of 18 times normal. Large numbers of glial cells but no neurons were observed in the lesioned CA fields at this time, suggesting that an increased calcium level was present in glial cells. This was confirmed by high-resolution elemental maps of the lesioned areas, which showed very high intracellular calcium concentrations in almost all glial cells. It is possible that the high intracellular calcium level could activate calcium- dependent enzymes, including calpain II and cytosolic phospholipase A2, shown to be expressed in reactive glial cells after kainate injections. In addition to calcium, an increase in iron content was also observed at the periphery of the glial scar at 4 weeks postinjection. Because free iron could catalyze the formation of free radicals, the late increase in iron content may be related to oxygen radical formation during neurodegeneration.
Source Title: Journal of Neurochemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/95665
ISSN: 00223042
DOI: 10.1046/j.1471-4159.1999.721574.x
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

40
checked on Aug 14, 2018

WEB OF SCIENCETM
Citations

34
checked on Aug 7, 2018

Page view(s)

48
checked on Jun 22, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.