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|Title:||Two-coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms|
|Authors:||Khor, E. |
|Citation:||Khor, E.,Ng, W.-F.,Loh, C.-S. (1998-09-05). Two-coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms. Biotechnology and Bioengineering 59 (5) : 635-639. ScholarBank@NUS Repository. https://doi.org/10.1002/(SICI)1097-0290(19980905)59:53.0.CO;2-8|
|Abstract:||Complex coacervation of alginate-chitosan and alginate-gelatin were used to develop two-coat systems for the encapsulation of Spathoglottis plicata seeds and protocorms (top-shaped structures formed after seed germination of orchids). Both the seeds and the protocorms could withstand the encapsulation treatments with high viability. About 54% of seeds and 40% of large protocorms (1.6-2.0 mm) were able to tolerate a 6-h desiccation treatment. However, viability of the small protocorms (0.7-0.9 mm) was greatly reduced if they were desiccated before encapsulation. Encapsulation after desiccation significantly increased the percentage viability of seeds and protocorms. Treatment with abscisic acid (ABA, 10-5 M) before desiccation and encapsulation resulted in high percentage viability in seeds and large protocorms whereas the small protocorms were found to be less tolerant to the treatments.|
|Source Title:||Biotechnology and Bioengineering|
|Appears in Collections:||Staff Publications|
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