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https://doi.org/10.1002/(SICI)1097-0290(19980905)59:53.0.CO;2-8
Title: | Two-coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms | Authors: | Khor, E. Ng, W.-F. Loh, C.-S. |
Keywords: | Artificial seed Complex coacervation Encapsulation Orchid Spathoglottis plicata Two-coat systems |
Issue Date: | 5-Sep-1998 | Citation: | Khor, E.,Ng, W.-F.,Loh, C.-S. (1998-09-05). Two-coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms. Biotechnology and Bioengineering 59 (5) : 635-639. ScholarBank@NUS Repository. https://doi.org/10.1002/(SICI)1097-0290(19980905)59:53.0.CO;2-8 | Abstract: | Complex coacervation of alginate-chitosan and alginate-gelatin were used to develop two-coat systems for the encapsulation of Spathoglottis plicata seeds and protocorms (top-shaped structures formed after seed germination of orchids). Both the seeds and the protocorms could withstand the encapsulation treatments with high viability. About 54% of seeds and 40% of large protocorms (1.6-2.0 mm) were able to tolerate a 6-h desiccation treatment. However, viability of the small protocorms (0.7-0.9 mm) was greatly reduced if they were desiccated before encapsulation. Encapsulation after desiccation significantly increased the percentage viability of seeds and protocorms. Treatment with abscisic acid (ABA, 10-5 M) before desiccation and encapsulation resulted in high percentage viability in seeds and large protocorms whereas the small protocorms were found to be less tolerant to the treatments. | Source Title: | Biotechnology and Bioengineering | URI: | http://scholarbank.nus.edu.sg/handle/10635/95367 | ISSN: | 00063592 | DOI: | 10.1002/(SICI)1097-0290(19980905)59:53.0.CO;2-8 |
Appears in Collections: | Staff Publications |
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