Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0021-9673(98)00561-5
Title: Selection and optimization of background electrolytes for simultaneous detection of small cations and organic acids by capillary electrophoresis with indirect photometry
Authors: Xiong, X.
Li, S.F.Y. 
Keywords: Background electrolyte composition
Food analysis
Fruit juices
Inorganic cations
Optimization
Organic acids
Soft drinks
Issue Date: 25-Sep-1998
Citation: Xiong, X., Li, S.F.Y. (1998-09-25). Selection and optimization of background electrolytes for simultaneous detection of small cations and organic acids by capillary electrophoresis with indirect photometry. Journal of Chromatography A 822 (1) : 125-136. ScholarBank@NUS Repository. https://doi.org/10.1016/S0021-9673(98)00561-5
Abstract: Background electrolytes (BGEs) containing two UV-absorbing probes were designed to separate and detect alkali metals and organic acids simultaneously. Imidazole, 1,2-dimethylimidazole, benzylamine, sulfosalicylic acid, trimellitic acid, and pyromellitic acid were tested as the cationic and anionic components of the BGE. By comparing the electrophoretic behavior and UV absorption characteristics of these compounds, it was found that the 1,2-dimethylimidazole/trimellitic acid combination was the most suitable BGE for the separation of alkali metals and organic acids. 18-Crown-6, added as an organic additive for resolving NH4 + and K+ peaks, had no influence on the separation and detection of the organic acids. Separation conditions were optimized by using a three-factor, three-level, face-centred central composite design with respect to three individual (electroosmotic flow, analysis time, and separation quality) and combined responses. The results obtained showed that proper selection of the BGEs and separation conditions are essential in the simultaneous detection of small cations and anions. Under optimal conditions, NH4 +, K+, Na+, Li+, ascorbate, sorbate, benzoate, lactate, acetate, succinate, malate, tartarate, maleate, malonate, perchlorate, and oxalate could be separated and detected within 6 min with detection limits ranging from 0.08 to 5 μg/ml. The newly developed method was successfully used to analyze three soft drinks, i.e., apple, orange, and grape juices. The results obtained showed that the present approach was simple, fast and could be applied to the analysis of real samples. Copyright (C) 1998 Elsevier Science B.V.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/94757
ISSN: 00219673
DOI: 10.1016/S0021-9673(98)00561-5
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