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Title: Profiles and α-amylase inhibition activity of proanthocyanidins in unripe Manilkara zapota (Chiku)
Authors: Wang, H.
Liu, T.
Song, L.
Huang, D. 
Keywords: α-amylase inhibitor
α-glucosidase inhibitor
Manilkara zapota
Issue Date: 28-Mar-2012
Source: Wang, H., Liu, T., Song, L., Huang, D. (2012-03-28). Profiles and α-amylase inhibition activity of proanthocyanidins in unripe Manilkara zapota (Chiku). Journal of Agricultural and Food Chemistry 60 (12) : 3098-3104. ScholarBank@NUS Repository.
Abstract: Proanthocyanidins in unripe Manilkara zapota (chiku) were isolated using solvent extraction followed by Sephadex LH-20 fractionation with a yield of 0.9%. HPLC analysis using a diol column revealed well-resolved oligomers ranging from dimer to hexamer. The majority of the proanthocyanidins are composed of higher-degree oligomers appearing as one large peak in the chromatogram. Analysis of the proanthocyanidins using LC/MS showed that (epi)gallocatechins were the dominant extension unit in the proanthocyanidins. In agreement with this result, thiolysis treatment of the proanthocyanidins using mercaptoacetic acid produced thioether derivatives of (epi)gallocatechins as the major product and (epi)gallocatechin gallate derivatives as the minor product. The mean of the degree of polymerization was estimated to be 9.0. From MALDI-TOF MS, B-type gallocatechin oligomers up to decamer could be detected. The unripe chiku proanthocyanidins are thus good starting material for preparation of (epi)gallocatechin derivatives. The proanthocyanidins was shown to inhibit α-amylase with an IC 50 value of 4.2 ± 0.2 μg/mL and inhibit α-glucosidase with an IC 50 of 16.6 ± 0.3 μg/mL. © 2012 American Chemical Society.
Source Title: Journal of Agricultural and Food Chemistry
ISSN: 00218561
DOI: 10.1021/jf204715q
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