Please use this identifier to cite or link to this item: https://doi.org/10.1021/jf204715q
Title: Profiles and α-amylase inhibition activity of proanthocyanidins in unripe Manilkara zapota (Chiku)
Authors: Wang, H.
Liu, T.
Song, L.
Huang, D. 
Keywords: α-amylase inhibitor
α-glucosidase inhibitor
gallocatechin
Manilkara zapota
proanthocyanidins
Issue Date: 28-Mar-2012
Citation: Wang, H., Liu, T., Song, L., Huang, D. (2012-03-28). Profiles and α-amylase inhibition activity of proanthocyanidins in unripe Manilkara zapota (Chiku). Journal of Agricultural and Food Chemistry 60 (12) : 3098-3104. ScholarBank@NUS Repository. https://doi.org/10.1021/jf204715q
Abstract: Proanthocyanidins in unripe Manilkara zapota (chiku) were isolated using solvent extraction followed by Sephadex LH-20 fractionation with a yield of 0.9%. HPLC analysis using a diol column revealed well-resolved oligomers ranging from dimer to hexamer. The majority of the proanthocyanidins are composed of higher-degree oligomers appearing as one large peak in the chromatogram. Analysis of the proanthocyanidins using LC/MS showed that (epi)gallocatechins were the dominant extension unit in the proanthocyanidins. In agreement with this result, thiolysis treatment of the proanthocyanidins using mercaptoacetic acid produced thioether derivatives of (epi)gallocatechins as the major product and (epi)gallocatechin gallate derivatives as the minor product. The mean of the degree of polymerization was estimated to be 9.0. From MALDI-TOF MS, B-type gallocatechin oligomers up to decamer could be detected. The unripe chiku proanthocyanidins are thus good starting material for preparation of (epi)gallocatechin derivatives. The proanthocyanidins was shown to inhibit α-amylase with an IC 50 value of 4.2 ± 0.2 μg/mL and inhibit α-glucosidase with an IC 50 of 16.6 ± 0.3 μg/mL. © 2012 American Chemical Society.
Source Title: Journal of Agricultural and Food Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/94625
ISSN: 00218561
DOI: 10.1021/jf204715q
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

30
checked on Dec 4, 2018

WEB OF SCIENCETM
Citations

29
checked on Dec 4, 2018

Page view(s)

50
checked on Dec 7, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.