Please use this identifier to cite or link to this item:
|Title:||Optimizing label-free DNA electrical detection on graphene platform|
|Authors:||Dubuisson, E. |
|Citation:||Dubuisson, E., Yang, Z., Loh, K.P. (2011-04-01). Optimizing label-free DNA electrical detection on graphene platform. Analytical Chemistry 83 (7) : 2452-2460. ScholarBank@NUS Repository. https://doi.org/10.1021/ac102431d|
|Abstract:||The anodized epitaxial graphene (EG) electrode demonstrates a high level of performance for electrochemical impedance as well as differential pulse voltammetry detection of immobilized DNA and free DNA, respectively, at solid-liquid interfaces. On the anodized EG surface, because of the presence of oxygen functionalities as well as π conjugated domains, the anchoring of the DNA probe can be achieved by either covalent grafting or noncovalent π-π stacking readily. The effect of different binding modes on the sensitivity of the impedimetric sensing was investigated. Equivalent circuit modeling shows that the sensitivity of EG to DNA hybridization is controlled by changes in the resistance of the molecular layer as well as the space charge layer. The linear dynamic detection range of EG for DNA oligonucleotides is in the range of 5.0 × 10-14 to 1 × 10-6 M. In addition, with the use of differential pulse voltammetry, single stranded DNA, fully complimentary DNA, as well as single nucleotide polymorphisms can be differentiated on anodized EG by monitoring the oxidation signals of individual nucleotide bases. © 2011 American Chemical Society.|
|Source Title:||Analytical Chemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 18, 2018
WEB OF SCIENCETM
checked on Oct 10, 2018
checked on Sep 28, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.