Please use this identifier to cite or link to this item: https://doi.org/10.1089/jmf.2010.1150
Title: Momordica charantia seed extract reduces pre-adipocyte viability, affects lactate dehydrogenase release, and lipid accumulation in 3T3-L1 cells
Authors: Popovich, D.G. 
Lee, Y.
Li, L.
Zhang, W.
Keywords: 3T3-L1 cells
adipogenesis
bitter melon
Momordica charantia seed
saponins
triterpenoids
Issue Date: 1-Mar-2011
Citation: Popovich, D.G., Lee, Y., Li, L., Zhang, W. (2011-03-01). Momordica charantia seed extract reduces pre-adipocyte viability, affects lactate dehydrogenase release, and lipid accumulation in 3T3-L1 cells. Journal of Medicinal Food 14 (3) : 201-208. ScholarBank@NUS Repository. https://doi.org/10.1089/jmf.2010.1150
Abstract: A triterpenoid containing bitter melon (Momordica charantia) seed (BMS) extract was found to reduce cultured 3T3-L1 cell viability. The 50% lethal concentration values were determined to be 0.78 ± 0.01 mg/mL at 24 hours, 0.69 ± 0.01 mg/mL at 48 hours, and 0.56 ± 0.02 mg/mL at 72 hours. 3T3-L1 cells were utilized as models of pre-adipocyte to adipocyte differentiation. BMS extract also caused a G2/M arrest in the cell cycle reducing cells by 23.9%, 37.7%, and 34.7% compared with the control after 72 hours of treatment at concentrations of 0.4, 0.5, and 0.6 mg/mL respectively. BMS extract did not increase the release of lactate dehydrogenase from 3T3-L1 cells, which was unexpected. Furthermore, BMS extract reduced lipid accumulation during differentiation from pre-adipocyte to adipocyte corresponding to reduction in overall triglyceride of 32.4% after 72 hours compared with untreated control cells. BMS is an underutilized agricultural commodity that may have potential for nutraceutical and functional food development. Copyright 2011, Mary Ann Liebert, Inc.
Source Title: Journal of Medicinal Food
URI: http://scholarbank.nus.edu.sg/handle/10635/94317
ISSN: 1096620X
DOI: 10.1089/jmf.2010.1150
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