Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2013.12.012
Title: Highly sensitive and quantitative human thrombospondin-1 detection by an M55 aptasensor and clinical validation in patients with atherosclerotic disease
Authors: Ji, K.
de Carvalho, L.P.
Bi, X.
Seneviratnankn, A.
Bhakoo, K.
Chan, M.
Yau Li, S.F. 
Keywords: Aptamer
Aptasensor
Atherosclerosis
Thrombospondin
Issue Date: 15-May-2014
Citation: Ji, K., de Carvalho, L.P., Bi, X., Seneviratnankn, A., Bhakoo, K., Chan, M., Yau Li, S.F. (2014-05-15). Highly sensitive and quantitative human thrombospondin-1 detection by an M55 aptasensor and clinical validation in patients with atherosclerotic disease. Biosensors and Bioelectronics 55 : 405-411. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2013.12.012
Abstract: Aptamer-based biosensors (aptasensor) are powerful tools for rapid and sensitive biomarker detection. In this study, we report a DNA aptamer probe evolved from cell-SELEX that can recognize thrombospondin-1 protein in human plasma samples. The KD value of the aptamer M55 binding to thrombospondin-1 was determined as 0.5±0.2μM with an R2 of 0.9144. A horseradish peroxidase-linked short oligo was complementarily bound onto the 3' end of the aptamer sequence to facilitate the 'smart' design of an M55-aptasensor for quantifying thrombospondin-1 protein in plasma samples. The limit of detection was 6.96fM. Thrombospondin-1 is a glycoprotein with multiple biological functions, including inflammation, platelet aggregation and endothelial cell apoptosis, and is involved in the pathology of atherosclerosis. In total, 118 plasma subjects were analyzed by using the aptasensor measurement with 1μL sample volume and 5min incubation time. The thrombospondin-1 concentrations in ST-Elevation Myocardial Infarction patients with severe atherosclerotic plaque burden were statistically significantly higher than in the healthy volunteers without atherosclerosis conditions, suggesting that thromboposnidn-1 is a potential plasma biomarker for atherosclerosis progression. © 2013 Elsevier B.V.
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/93951
ISSN: 09565663
DOI: 10.1016/j.bios.2013.12.012
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