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|Title:||Development of a biomimetic nanoporous membrane for the selective transport of charged proteins|
|Citation:||Nguyen, B.T.T., Ting, E.Z.C., Toh, C.-S. (2008-09-01). Development of a biomimetic nanoporous membrane for the selective transport of charged proteins. Bioinspiration and Biomimetics 3 (3) : -. ScholarBank@NUS Repository. https://doi.org/10.1088/1748-3182/3/3/035008|
|Abstract:||We report the use of a micrometer-thick platinum-coated nanoporous membrane for the separation of differently charged proteins. A high field strength of about 25 kV m-1 was applied, using very low transmembrane potentials of ±1.5 V between the platinum-coated membranes. The system mimics the cell membrane function of facilitated transport for specific solutes. The selectivity for Lys:BSA:Mb in a mixed protein solution could be tuned readily between the flux ratios of 2:2:1 and 96:1:12 respectively, by simple variation of the transmembrane potentials from +1.5 V to -1.5 V. The experimental fluxes agreed closely with calculated fluxes derived from a simple electrophoresis- potential shielding model at favourable transmembrane potentials. © 2008 IOP Publishing Ltd.|
|Source Title:||Bioinspiration and Biomimetics|
|Appears in Collections:||Staff Publications|
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