Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.talanta.2014.03.058
Title: Determination of total thyroxine in human serum by hollow fiber liquid-phase microextraction and liquid chromatography-tandem mass spectrometry
Authors: Yong, S.
Chen, Y.
Lee, T.K.
Lee, H.K. 
Keywords: Hollow fiber liquid-phase microextraction
Human serum
Liquid chromatography-tandem mass spectrometry
Total thyroxine
Issue Date: 1-Aug-2014
Citation: Yong, S., Chen, Y., Lee, T.K., Lee, H.K. (2014-08-01). Determination of total thyroxine in human serum by hollow fiber liquid-phase microextraction and liquid chromatography-tandem mass spectrometry. Talanta 126 : 163-169. ScholarBank@NUS Repository. https://doi.org/10.1016/j.talanta.2014.03.058
Abstract: Determination of total thyroxine in human serum using hollow fiber liquid-phase microextraction (HF-LPME) has been accomplished for the first time. HF-LPME serves as an inexpensive sample pretreatment and the cleanup method that is nearly solvent-free. Thyroxine was extracted through a water immiscible organic solvent immobilized in the wall pores of a polypropylene hollow fiber into 20 μl of an aqueous acceptor phase inside the lumen of the hollow fiber. This technique produced extracts that had comparable cleanness with those obtained using solid-phase extraction (SPE). Serum samples with endogenous thyroxine were spiked with isotopically-labeled thyroxine and analyzed by liquid chromatography-tandem mass spectrometry after HF-LPME extraction. Extraction parameters including the organic phase, acid/base concentration of acceptor phase, stirring speed and extraction time were optimized. The calibration range was found to be linear over 1-1000 ng/g with the limit of detection (LOD) of 0.3 -ng/g. For quantification of total thyroxine in human serum, 6 subsamples were prepared and the results indicated very good precision with a relative standard deviation of
Source Title: Talanta
URI: http://scholarbank.nus.edu.sg/handle/10635/93563
ISSN: 00399140
DOI: 10.1016/j.talanta.2014.03.058
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