Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0021-9673(98)00279-9
Title: Determination of glycyrrhizic acid and 18-β-glycyrrhetinic acid in biological fluids by micellar electrokinetic chromatography
Authors: Wang, P.
Li, S.F.Y. 
Lee, H.K. 
Keywords: Glycyrrhetinic acid
Glycyrrhizic acid
Methyltestosterone
Issue Date: 19-Jun-1998
Citation: Wang, P., Li, S.F.Y., Lee, H.K. (1998-06-19). Determination of glycyrrhizic acid and 18-β-glycyrrhetinic acid in biological fluids by micellar electrokinetic chromatography. Journal of Chromatography A 811 (1-2) : 219-224. ScholarBank@NUS Repository. https://doi.org/10.1016/S0021-9673(98)00279-9
Abstract: A micellar electrokinetic chromatographic technique for determining glycyrrhizic acid (GZA) and 18β-glycyrrhetinic acid (GRA) in human plasma and urine was developed. Sample clean-up and analyte concentration were carded out by solid-phase extraction (with C18 sorbent). The buffer solution used for MEKC contained 20 mM sodium dihydrogenphosphate, 20 mM sodium tetraborate, 20 mM tetrabutylammonium bromide and 50 mM sodium dodecylsulphate, at a pH of 8.7. Good linearities for both GZA and GRA in plasma and urine were obtained. The recoveries of the method were in the range 86.5 to 107%. The detection limits for GZA in urine (0.5 ml) and plasma (1 ml) were 1.6 μg ml-1 and 0.8 μg ml-1, respectively, while for GRA in urine (0.5 ml) and plasma (1 ml) were 2 μg ml-1 and 1 μg ml-1, respectively.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/93550
ISSN: 00219673
DOI: 10.1016/S0021-9673(98)00279-9
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