Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/93545
Title: Determination of critical micelle concentrations and aggregation numbers by fluorescence correlation spectroscopy: Aggregation of a lipopolysaccharide
Authors: Yu, L.
Tan, M.
Ho, B.
Ding, J.L. 
Wohland, T. 
Keywords: Critical micelle concentration
Fluorescence correlation spectroscopy
Lipopolysaccharide
Issue Date: 18-Jan-2006
Citation: Yu, L., Tan, M., Ho, B., Ding, J.L., Wohland, T. (2006-01-18). Determination of critical micelle concentrations and aggregation numbers by fluorescence correlation spectroscopy: Aggregation of a lipopolysaccharide. Analytica Chimica Acta 556 (1) : 216-225. ScholarBank@NUS Repository.
Abstract: Fluorescence correlation spectroscopy (FCS) is often used to determine the mass or radius of a particle by using the dependence of the diffusion coefficient on the mass and shape. In this article we discuss how the particle size of aggregates can be measured by using the concentration dependence of the amplitude of the autocorrelation function (ACF) instead of the temporal decay. We titrate a solution of aggregates or micelles with a fluorescent label that possesses a high affinity for these structures and measure the changes in the amplitude of the ACF. We develop the theory describing the change of the ACF amplitude with increasing concentrations of labels and use it to fit experimental data. It is shown how this method can determine the aggregation number and critical micelle concentration of a standard detergent nonaethylene glycol monododecyl ether (C12E9) and a lipopolysaccharide (LPS: Escherichia coli 0111:B4). © 2005 Elsevier B.V. All rights reserved.
Source Title: Analytica Chimica Acta
URI: http://scholarbank.nus.edu.sg/handle/10635/93545
ISSN: 00032670
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

65
checked on Feb 28, 2018

WEB OF SCIENCETM
Citations

63
checked on Apr 23, 2018

Page view(s)

44
checked on Nov 9, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.