Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.aca.2010.12.007
Title: Application of porous membrane protected micro-solid-phase-extraction combined with gas chromatography-mass spectrometry for the determination of estrogens in ovarian cyst fluid samples
Authors: Kanimozhi, S.
Basheer, C.
Narasimhan, K.
Liu, L.
Koh, S.
Xue, F. 
Choolani, M.
Lee, H.K. 
Keywords: Cancer risk
Cyst fluids
Estrogens
Micro-solid-phase-extraction
Ovarian cancer
Issue Date: 14-Feb-2011
Citation: Kanimozhi, S., Basheer, C., Narasimhan, K., Liu, L., Koh, S., Xue, F., Choolani, M., Lee, H.K. (2011-02-14). Application of porous membrane protected micro-solid-phase-extraction combined with gas chromatography-mass spectrometry for the determination of estrogens in ovarian cyst fluid samples. Analytica Chimica Acta 687 (1) : 56-60. ScholarBank@NUS Repository. https://doi.org/10.1016/j.aca.2010.12.007
Abstract: A cost effective and environmentally friendly extraction technique using porous membrane protected micro-solid phase extraction (μ-SPE) is described for the extraction of estrogens in cyst fluid samples obtained from cancer patients. A sorbent (ethylsilane (C2) modified silica) (20mg) was packed in a porous polypropylene envelope (2cm×1.5cm) whose edges were heat sealed to secure the contents. The μ-SPE device was conditioned with acetone and placed in a stirred (1:5) diluted cyst fluid sample solution (10mL) to extract estrogens for 60min. After extraction, the analytes were desorbed and simultaneously derivatized with a 5:1 mixture of acetone and N,O-bis(trimethylsilyl)-trifluoroacetamide. The extract (2μL) was analyzed by gas chromatography-mass spectrometry. Various extraction, desorption and derivatization conditions were optimized for μ-SPE. With this simple technique, low limits of detection of between 9 and 22ngL-1 and linear range from the detection limits up to 50μgL-1 were achieved. The optimized method was used to extract estrogens from cyst fluid samples obtained from patients with malignant and benign ovarian tumors. © 2010 Elsevier B.V.
Source Title: Analytica Chimica Acta
URI: http://scholarbank.nus.edu.sg/handle/10635/93129
ISSN: 00032670
DOI: 10.1016/j.aca.2010.12.007
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