Please use this identifier to cite or link to this item:
|Title:||A novel strategy for rapid real-time chiral discrimination of enantiomers using serum albumin functionalized QCM biosensor|
|Citation:||Su, W.C., Zhang, W.G., Zhang, S., Fan, J., Yin, X., Luo, M.L., Ng, S.C. (2009-10-15). A novel strategy for rapid real-time chiral discrimination of enantiomers using serum albumin functionalized QCM biosensor. Biosensors and Bioelectronics 25 (2) : 488-492. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2009.06.040|
|Abstract:||A novel and effective method has been developed for chiral discrimination using a quartz crystal microbalance (QCM) biosensor with self-assembled bovine serum albumin (BSA) or human serum albumin (HSA). The successfully constructed QCM chiral biosensors exhibited rapid and real-time enantioselective recognition. The QCM chiral discrimination factor (αQCM) can be calculated through resonance frequency shifts in response to five pairs of enantiomers. Moreover, the interactions between these ten enantiomers and two serum albumins (SA) were investigated in detail by means of ultraviolet-visible (UV-vis) and fluorescence (FL) spectra. The results indicated that the discrimination ability were quite different between BSA and HSA. R,S-1-(3-Methoxyphenyl)ethylamine (R,S-3-MPEA) and R,S-1-(4-methoxyphenyl)ethylamine (R,S-4-MPEA) can be easily differentiated by the BSA sensor, while the selectivity of the HSA sensor for R,S-tetrahydronaphthylamine (R,S-TNA), R,S-2-octanol (R,S-2-OT) and R,S-methyl lactate (R,S-MEL) was higher than that of the BSA sensor. The UV and FL spectra indicated the formation of a complex between SA and enantiomers and strong fluorescence quenching through static quenching mechanism. The in-depth study demonstrated that the calculated UV/FL discrimination factors (αUV and αFL) were consistent with the QCM experimental results (αQCM). © 2009 Elsevier B.V. All rights reserved.|
|Source Title:||Biosensors and Bioelectronics|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 15, 2018
WEB OF SCIENCETM
checked on Oct 8, 2018
checked on Oct 5, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.