Please use this identifier to cite or link to this item: https://doi.org/10.1002/chir.22074
Title: A new biosensor for chiral recognition using goat and rabbit serum albumin self-assembled quartz crystal microbalance
Authors: Chen, W.-J.
Zhang, S. 
Zhang, W.-G.
Fan, J.
Yin, X.
Zheng, S.-R.
Su, W.-C.
Zhang, Z.
Hong, T.
Keywords: biosensor
chiral recognition
QCM
serum albumin
UV/FL spectra
Issue Date: Oct-2012
Source: Chen, W.-J., Zhang, S., Zhang, W.-G., Fan, J., Yin, X., Zheng, S.-R., Su, W.-C., Zhang, Z., Hong, T. (2012-10). A new biosensor for chiral recognition using goat and rabbit serum albumin self-assembled quartz crystal microbalance. Chirality 24 (10) : 804-809. ScholarBank@NUS Repository. https://doi.org/10.1002/chir.22074
Abstract: Quartz crystal microbalance (QCM) biosensor was used for the chiral recognition of five pairs of enantiomers by using goat serum albumin (GSA) and rabbit serum albumin (RbSA) as chiral selectors. Serum albumin (SA) was immobilized on the QCM through the self-assembled monolayer technique, and the surface concentration of GSA and RbSA were 8.8 × 10-12 mol cm-2 and 1.2 × 10-11 mol cm-2, respectively. The QCM biosensors showed excellent sensitivity and selectivity. Meanwhile, the chiral recognition of SA sensors was quite species dependent. There were differences between GSA and RbSA sensors in the ability and the preference of chiral recognition. To R,S-1,2,3,4-tetrahydro-1-naphthylamine (R,S-1-TNA), R,S-1-(4-methoxyphenyl)ethylamine (R,S-4-MPEA), and R,S-1-(3-methoxyphenyl)ethylamine (R,S-3-MPEA), the preference of the stereoselective SA-drug binding of the two kinds of SA sensors were consistent. However, to R,S-2-octanol (R, S-2-OT) and R,S-methyl lactate (R,S-MEL), the two kinds of SA sensors had opposite chiral recognition preference. Moreover, the interactions of SA and the five pairs of enantiomers have been further investigated through ultraviolet (UV) and fluorescent (FL) spectra. The UV/FL results were in accordance with the consequence of QCM. Copyright © 2012 Wiley Periodicals, Inc.
Source Title: Chirality
URI: http://scholarbank.nus.edu.sg/handle/10635/92960
ISSN: 08990042
DOI: 10.1002/chir.22074
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