Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2014.04.023
Title: A highly sensitive microRNA biosensor based on hybridized microRNA-guided deposition of polyaniline
Authors: Deng, H.
Shen, W.
Ren, Y.
Gao, Z. 
Keywords: DNAzyme
Electrochemical impedance
Gold electrode
Microrna
Polyaniline
Issue Date: 15-Oct-2014
Source: Deng, H., Shen, W., Ren, Y., Gao, Z. (2014-10-15). A highly sensitive microRNA biosensor based on hybridized microRNA-guided deposition of polyaniline. Biosensors and Bioelectronics 60 : 195-200. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2014.04.023
Abstract: On the basis of hybridized microRNA (miRNA)-guided deposition of polyaniline (PAn), a highly sensitive impedimetric miRNA biosensor is developed in this report. Briefly, a gold electrode coated with charge neutral peptide nucleic acid (PNA) capture probes (CPs) is first hybridized to a target miRNA. After a very brief rinsing the hybridized electrode is incubated in pH 3.0 of 0.10M potassium phosphate buffer-based cocktail containing aniline, H2O2, and a G-qudraplex-hemin DNAzyme. The DNAzyme catalyzes the polymerization of aniline and the hybridized miRNA strands guide the deposition of PAn, thus resulting in the formation of a thin PAn film on the biosensor surface. Electron-transfer impeding power of the PAn film in alkaline medium is utilized to determine the concentration of the target miRNA. Under optimized experimental conditions, 0.50fM target miRNA is successfully detected. Excellent mismatch discrimination capability of the biosensor was observed largely due to the excellent hybridization selectivity of the PNA CPs. Attempts were made in profiling miRNAs in total RNA samples extracted from cancer cells and blood. © 2014 Elsevier B.V.
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/92947
ISSN: 18734235
DOI: 10.1016/j.bios.2014.04.023
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