Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0956-5663(02)00149-5
Title: Surface functionalization of polypyrrole film with glucose oxidase and viologen
Authors: Cen, L. 
Neoh, K.G. 
Kang, E.T. 
Keywords: Covalent immobilization
Glucose oxidase
Polypyrrole
Surface functionalization
Viologen
Issue Date: 1-Apr-2003
Citation: Cen, L., Neoh, K.G., Kang, E.T. (2003-04-01). Surface functionalization of polypyrrole film with glucose oxidase and viologen. Biosensors and Bioelectronics 18 (4) : 363-374. ScholarBank@NUS Repository. https://doi.org/10.1016/S0956-5663(02)00149-5
Abstract: A surface modification technique was developed for the functionalization of polypyrrole (PPY) film with glucose oxidase (GOD) and viologen moieties. The PPY film was first graft copolymerized with acrylic acid (AAc) and GOD was then covalently immobilized through the amide linkage formation between the amino groups of the GOD and the carboxyl groups of the grafted AAc polymer chains in the presence of a water-soluble carbodiimide. Viologen moieties could also be attached to the PPY film via graft-copolymerization of vinyl benzyl chloride with the PPY film surface followed by reaction with 4,4′-bipyridine and α,α′-dichloro-p-xylene. X-ray photoelectron spectroscopy (XPS) was used to characterize the PPY films after each surface modification step. Increasing the AAc graft concentration would allow a greater amount of GOD to be immobilized but this would decrease the electrical conductivity of the PPY film. The activity of the immobilized GOD was compared with that of free GOD and the kinetic effects were also studied. The immobilized GOD was found to be less sensitive to temperature deactivation as compared to the free GOD. The results showed that the covalent immobilization technique offers advantages over the technique involving the entrapment of GOD in PPY films during electropolymerization. The presence of viologen in the vicinity of the immobilized GOD also enabled the GOD-catalyzed oxidation of glucose to proceed under UV irradiation in the absence of O2. © 2002 Elsevier Science B.V. All rights reserved.
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/92364
ISSN: 09565663
DOI: 10.1016/S0956-5663(02)00149-5
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

49
checked on Oct 19, 2018

WEB OF SCIENCETM
Citations

48
checked on Oct 2, 2018

Page view(s)

30
checked on Sep 21, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.