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|Title:||Optimisation of the dehydrogenase assay for measurement of indigenous microbial activity in beach sediments contaminated with petroleum|
|Citation:||Mathew, M., Obbard, J.P. (2001). Optimisation of the dehydrogenase assay for measurement of indigenous microbial activity in beach sediments contaminated with petroleum. Biotechnology Letters 23 (3) : 227-230. ScholarBank@NUS Repository.|
|Abstract:||A dehydrogenase enzyme assay has been developed to measure microbial activity in petroleum-contaminated beach sediments. The assay, using 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazoliumchloride (INT) as the substrate provides a reliable measurement of overall indigenous microbiological activity in beach sediments containing ∼7% (w/w) oil. The method has been effectively used in field studies (Mathew et al. 1999) to monitor microbial activity in conjunction with the biodegradation of petroleum hydrocarbons.|
|Source Title:||Biotechnology Letters|
|Appears in Collections:||Staff Publications|
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