Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/92191
Title: Optimisation of the dehydrogenase assay for measurement of indigenous microbial activity in beach sediments contaminated with petroleum
Authors: Mathew, M.
Obbard, J.P. 
Keywords: Beach sediments
Dehydrogenase
Hydrocarbon
Redox measurements
Tetrazolium salts
Issue Date: 2001
Citation: Mathew, M., Obbard, J.P. (2001). Optimisation of the dehydrogenase assay for measurement of indigenous microbial activity in beach sediments contaminated with petroleum. Biotechnology Letters 23 (3) : 227-230. ScholarBank@NUS Repository.
Abstract: A dehydrogenase enzyme assay has been developed to measure microbial activity in petroleum-contaminated beach sediments. The assay, using 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazoliumchloride (INT) as the substrate provides a reliable measurement of overall indigenous microbiological activity in beach sediments containing ∼7% (w/w) oil. The method has been effectively used in field studies (Mathew et al. 1999) to monitor microbial activity in conjunction with the biodegradation of petroleum hydrocarbons.
Source Title: Biotechnology Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/92191
ISSN: 01415492
Appears in Collections:Staff Publications

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