Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/92027
Title: High density of immobilized galactose ligand enhances hepatocyte attachment and function
Authors: Yin, C.
Ying, L.
Zhang, P.-C.
Zhuo, R.-X.
Kang, E.-T. 
Leong, K.W.
Mao, H.-Q.
Keywords: Function maintenance
Galactosylated surface
Hepatocyte
Immobilization
Ligand
Issue Date: 15-Dec-2003
Source: Yin, C.,Ying, L.,Zhang, P.-C.,Zhuo, R.-X.,Kang, E.-T.,Leong, K.W.,Mao, H.-Q. (2003-12-15). High density of immobilized galactose ligand enhances hepatocyte attachment and function. Journal of Biomedical Materials Research - Part A 67 (4) : 1093-1104. ScholarBank@NUS Repository.
Abstract: Galactosylated surface is an attractive substrate for hepatocyte culture because of the specific interaction between the galactose ligand and the asialoglycoprotein receptor on hepatocytes. In this study, we described a scheme to achieve high density of immobilized galactose ligands on polyethylene terephthalate (PET) surface by first surface-grafting polyacrylic acid on plasma-pretreated PET film under UV irradiation, followed by conjugation of a galactose derivative (1-O-(6′-aminohexyl)-D-galactopyranoside) to the grafted polyacrylic acid chains. A high galactose density of 513 nmol/cm 2 on the PET surface was used in this study to investigate the behavior of cultured hepatocyte. This engineered substrate showed high affinity to fluorescein isothiocyanate-lectin binding. Primary rat hepatocytes, when seeded at a density of 2 x 105 cells/cm2, attached to the galactosylated PET substrate at a similar efficiency compared with collagen-coated substrate. The hepatocytes spontaneously formed aggregates 1 day after cell seeding and showed better maintenance of albumin secretion and urea synthesis functions than those cultured on collagen-coated surface. © 2003 Wiley Periodicals, Inc.
Source Title: Journal of Biomedical Materials Research - Part A
URI: http://scholarbank.nus.edu.sg/handle/10635/92027
ISSN: 00219304
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

25
checked on Feb 24, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.