Please use this identifier to cite or link to this item:
|Title:||Reducing cofactors contribute to the increase of butanol production by a wild-type Clostridium sp. strain BOH3|
|Source:||Li, T., Yan, Y., He, J. (2014-03). Reducing cofactors contribute to the increase of butanol production by a wild-type Clostridium sp. strain BOH3. Bioresource Technology 155 : 220-228. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2013.12.089|
|Abstract:||Availability of reducing factors (e.g., NADH and NADPH) plays an important role in improving the efficacy of products conversion in cofactor-dependent production systems. In this study, nicotinic acid (NA), the precursor of NADH and NADPH, was supplemented to the growth medium of a wild-type Clostridium sp. strain BOH3. Results showed that the addition of precursor NA to the medium led to a significant increase in the levels of NADH and NADPH. Meanwhile, a maximal cell growth rate and butanol generation rate were reached by applying a two-stage pH-shift strategy, achieving 18.7. g/L butanol with a yield of 24.6% and a productivity of 0.26. g/L. h. The metabolic patterns were shifted towards more reduced metabolites as reflected by higher butanol-to-acetone ratio (11%) and butanol-to-acid ratio (292%). Redistributing metabolic flux to butanol via manipulations of reducing cofactor and pH shift could become an alternative tool to realize metabolic engineering goals. © 2013 Elsevier Ltd.|
|Source Title:||Bioresource Technology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 14, 2018
WEB OF SCIENCETM
checked on Jan 22, 2018
checked on Feb 19, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.