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Title: Reducing cofactors contribute to the increase of butanol production by a wild-type Clostridium sp. strain BOH3
Authors: Li, T.
Yan, Y.
He, J. 
Keywords: Butanol
Carbon flux
Clostridium sp.
PH shift
Reducing cofactor
Issue Date: Mar-2014
Citation: Li, T., Yan, Y., He, J. (2014-03). Reducing cofactors contribute to the increase of butanol production by a wild-type Clostridium sp. strain BOH3. Bioresource Technology 155 : 220-228. ScholarBank@NUS Repository.
Abstract: Availability of reducing factors (e.g., NADH and NADPH) plays an important role in improving the efficacy of products conversion in cofactor-dependent production systems. In this study, nicotinic acid (NA), the precursor of NADH and NADPH, was supplemented to the growth medium of a wild-type Clostridium sp. strain BOH3. Results showed that the addition of precursor NA to the medium led to a significant increase in the levels of NADH and NADPH. Meanwhile, a maximal cell growth rate and butanol generation rate were reached by applying a two-stage pH-shift strategy, achieving 18.7. g/L butanol with a yield of 24.6% and a productivity of 0.26. g/L. h. The metabolic patterns were shifted towards more reduced metabolites as reflected by higher butanol-to-acetone ratio (11%) and butanol-to-acid ratio (292%). Redistributing metabolic flux to butanol via manipulations of reducing cofactor and pH shift could become an alternative tool to realize metabolic engineering goals. © 2013 Elsevier Ltd.
Source Title: Bioresource Technology
ISSN: 09608524
DOI: 10.1016/j.biortech.2013.12.089
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