Please use this identifier to cite or link to this item: https://doi.org/10.1007/s12033-010-9304-3
Title: Simultaneous targeting of requiem & Alg-2 in Chinese hamster ovary cells for improved recombinant protein production
Authors: Lim, Y.
Mantalaris, A.
Yap, M.G.S. 
Wong, D.C.F.
Keywords: alg-2
Apoptosis
Cell engineering
CHO
Mammalian cell culture
Requiem
Issue Date: Nov-2010
Citation: Lim, Y., Mantalaris, A., Yap, M.G.S., Wong, D.C.F. (2010-11). Simultaneous targeting of requiem & Alg-2 in Chinese hamster ovary cells for improved recombinant protein production. Molecular Biotechnology 46 (3) : 301-307. ScholarBank@NUS Repository. https://doi.org/10.1007/s12033-010-9304-3
Abstract: Apoptosis is known to be the main cause of cell death in the bioreactor environment, leading to the loss of recombinant protein productivity. In a previous study, transcriptional profiling was used to identify and target four early apoptosis-signaling genes: FADD, FAIM, Alg-2, and Requiem. The resulting cell lines had increased viable cell numbers and extended culture viability, which translated to increased protein productivity. Combinatorial targeting of two genes simultaneously has previously been shown to be more effective than targeting one gene alone. In this study, we sought to determine if targeting Requiem and Alg-2 was more effective than targeting Requiem alone. We found that targeting Requiem and Alg-2 did not result in extended culture viability, but resulted in an increase in maximum viable cell numbers and cumulative IVCD under fed-batch conditions. This in turn led to an approximately 1.5-fold increase in recombinant protein productivity. © 2010 Springer Science+Business Media, LLC.
Source Title: Molecular Biotechnology
URI: http://scholarbank.nus.edu.sg/handle/10635/90161
ISSN: 10736085
DOI: 10.1007/s12033-010-9304-3
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

1
checked on Sep 20, 2018

WEB OF SCIENCETM
Citations

1
checked on Sep 10, 2018

Page view(s)

28
checked on Aug 10, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.