Please use this identifier to cite or link to this item:
https://doi.org/10.1002/btpr.322
DC Field | Value | |
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dc.title | Regulation of XBP-1 signaling during transient and stable recombinant protein production in CHO cells | |
dc.contributor.author | Ku, S.C.Y. | |
dc.contributor.author | Toh, P.C. | |
dc.contributor.author | Lee, Y.Y. | |
dc.contributor.author | Chusainow, J. | |
dc.contributor.author | Yap, M.G.S. | |
dc.contributor.author | Chao, S.-H. | |
dc.date.accessioned | 2014-10-09T07:00:18Z | |
dc.date.available | 2014-10-09T07:00:18Z | |
dc.date.issued | 2010-03 | |
dc.identifier.citation | Ku, S.C.Y., Toh, P.C., Lee, Y.Y., Chusainow, J., Yap, M.G.S., Chao, S.-H. (2010-03). Regulation of XBP-1 signaling during transient and stable recombinant protein production in CHO cells. Biotechnology Progress 26 (2) : 517-526. ScholarBank@NUS Repository. https://doi.org/10.1002/btpr.322 | |
dc.identifier.issn | 87567938 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/90009 | |
dc.description.abstract | X-box binding protein 1 (XBP-1) is a key regulator of cellular unfolded protein response (UPR). The spliced isoform of XBP-1, XBP-1S, is a transcription activator, which is expressed only when UPR is induced. However, the impact of recombinant protein production on the regulation of XBP-1 signaling in CHO cells is not well understood. In this report, we cloned the Chinese hamster XBP-1 homolog to aid the investigation of the interplay between protein productivity, culture conditions, and endogenous XBP-1 signaling in CHO cells. Interestingly, expression of XBP-1S is detected in the non-producing and unstressed CHO-K1 cells. Transient expression of recombinant erythropoietin reveals a positive correlation between XBP-1 mRNA abundance and protein production level. However, such a correlation is not observed in batch cultivation of stable producing cell lines. The increased XBP-1 splicing is detected in late-phase cultures, suggesting that induction of XBP-1S may be a result of nutrient limitations or other environmental stresses rather than that of increased intracellular accumulation of recombinant proteins. Our data suggest that XBP-1 is a key determinant for the secretory capacity of CHO cells. Understanding its dynamic regulation hence provides a rational basis for cellular engineering strategies to improve recombinant protein secretion. © 2009 American Institute of Chemical Engineers Biotechnol. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/btpr.322 | |
dc.source | Scopus | |
dc.subject | CHO | |
dc.subject | Recombinant protein production | |
dc.subject | UPR | |
dc.subject | XBP-1 | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1002/btpr.322 | |
dc.description.sourcetitle | Biotechnology Progress | |
dc.description.volume | 26 | |
dc.description.issue | 2 | |
dc.description.page | 517-526 | |
dc.description.coden | BIPRE | |
dc.identifier.isiut | 000276985400023 | |
Appears in Collections: | Staff Publications |
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