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Title: Pyrene-based water dispersible orange emitter for one- and two-photon fluorescence cellular imaging
Authors: Wang, G.
Geng, J.
Zhang, X.
Cai, L. 
Ding, D. 
Li, K.
Wang, L.
Lai, Y.-H. 
Liu, B. 
Issue Date: Sep-2012
Source: Wang, G., Geng, J., Zhang, X., Cai, L., Ding, D., Li, K., Wang, L., Lai, Y.-H., Liu, B. (2012-09). Pyrene-based water dispersible orange emitter for one- and two-photon fluorescence cellular imaging. Polymer Chemistry 3 (9) : 2464-2470. ScholarBank@NUS Repository.
Abstract: A water-dispersible orange emitter (Pyrene4BTF-PEG-TAT) containing pyrene as the donor core and benzothiadiazole as the acceptor is synthesized through click chemistry for one- and two-photon fluorescence imaging application. Pyrene4BTF-PEG-TAT has an emission maximum at 586 nm in water with a large Stokes shift of 181 nm. It self-assembles into nanoparticles with diameters of ∼190 nm in water. The nanoparticles have a fluorescence quantum yield of 0.11 and a two-photon absorption (TPA) cross-section of 512 GM at 820 nm based on molecule. Pyrene4BTF-PEG-TAT could be effectively internalized by HeLa cells to show bright red fluorescence upon both one- and two-photon excitation. The successful demonstration of the first water-dispersible pyrene-based TPA material will trigger more research on developing red emitters for bioimaging applications. © 2012 The Royal Society of Chemistry.
Source Title: Polymer Chemistry
ISSN: 17599954
DOI: 10.1039/c2py20271a
Appears in Collections:Staff Publications

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