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https://scholarbank.nus.edu.sg/handle/10635/89956
DC Field | Value | |
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dc.title | Proteins combination on PHBV microsphere scaffold to regulate Hep3B cells activity and functionality: A model of liver tissue engineering system | |
dc.contributor.author | Xin, H.Z. | |
dc.contributor.author | Seng, K.G. | |
dc.contributor.author | Wang, C.-H. | |
dc.contributor.author | Tong, Y.W. | |
dc.date.accessioned | 2014-10-09T06:59:39Z | |
dc.date.available | 2014-10-09T06:59:39Z | |
dc.date.issued | 2007-11-01 | |
dc.identifier.citation | Xin, H.Z., Seng, K.G., Wang, C.-H., Tong, Y.W. (2007-11-01). Proteins combination on PHBV microsphere scaffold to regulate Hep3B cells activity and functionality: A model of liver tissue engineering system. Journal of Biomedical Materials Research - Part A 83 (3) : 606-616. ScholarBank@NUS Repository. | |
dc.identifier.issn | 15493296 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/89956 | |
dc.description.abstract | The synergistic effects of extracellular matrix (ECM) protein combinations on Hep3B cell proliferation and functions are studied herein. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) microspheres were covalently conjugated with three types of proteins, collagen (type I), laminin, and fibronectin, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide cross linkers. Successful conjugations of protein molecules were verified by the presence of nitrogen peaks in X-ray photoelectron spectroscopy. The densities of grafted proteins were quantified using Micro-BCA kit. A human hepatoma cell line, Hep3B, was then cultured in vitro on the ECM proteins-modified microspheres for 2 weeks. Cell proliferation was estimated using MTT method, and two hepatic functions, albumin secretion and P-450 activity, were evaluated using ELISA and EROD assays, respectively. The results indicated that combination of the three ECM proteins on microsphere surfaces has a significant effect on the proliferation of Hep3B cells, thus better mimicking the in vivo environment for liver tissue engineering. © 2007 Wiley Periodicals, Inc. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/jbm.a.31257 | |
dc.source | Scopus | |
dc.subject | Liver tissue engineering | |
dc.subject | Microsphere | |
dc.subject | PHBV | |
dc.subject | Protein | |
dc.subject | Surface conjugation | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.sourcetitle | Journal of Biomedical Materials Research - Part A | |
dc.description.volume | 83 | |
dc.description.issue | 3 | |
dc.description.page | 606-616 | |
dc.description.coden | JBMRC | |
dc.identifier.isiut | 000250742700004 | |
Appears in Collections: | Staff Publications |
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