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|Title:||Production of a chiral alcohol, 1-(3,4-dihydroxyphenyl) ethanol, by mushroom tyrosinase|
|Citation:||Brooks, S.J., Nikodinovic, J., Martin, L., Doyle, E.M., O'Sullivan, T., Guiry, P.J., Coulombel, L., Li, Z., O'Connor, K.E. (2013-05). Production of a chiral alcohol, 1-(3,4-dihydroxyphenyl) ethanol, by mushroom tyrosinase. Biotechnology Letters 35 (5) : 779-783. ScholarBank@NUS Repository. https://doi.org/10.1007/s10529-013-1148-z|
|Abstract:||1-(3,4-Dihydroxyphenyl) ethanol was produced biocatalytically for the first time using mushroom tyrosinase. 4-Ethylphenol at 1 mM was consumed over 12 min giving 0. 23 mM 4-ethylcatechol and 0. 36 mM (R/S)-1-(3,4-dihydroxyphenyl) ethanol (ee 0. 5 %). Mushroom tyrosinase consumed 4-ethylphenol at 6. 7 μmol min-1 mg protein-1 while the rates of formation of 4-ethylcatechol and 1-(3,4-dihydroxyphenyl) ethanol were 1. 1 and 1. 9 μmol min-1 mg protein-1. Addition of the ascorbic acid, as a reducing agent to biotransformation reactions, increased 4-ethylcatechol formation by 340 %. However, accumulation of 1-(3,4-dihydroxyphenyl) ethanol was not observed in the presence of ascorbic acid. While the 1-(3,4-dihydroxyphenyl) ethanol was racemic, it is the first chiral product produced by tyrosinase starting from a non-chiral substrate. © 2013 Springer Science+Business Media Dordrecht.|
|Source Title:||Biotechnology Letters|
|Appears in Collections:||Staff Publications|
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