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|Title:||Phalloidin-functionalized hyperbranched conjugated polyelectrolyte for filamentous actin imaging in living Hela cells|
fluorescence living cell imaging
hyperbranched conjugated polymer
|Citation:||Li, K., Pu, K.-Y., Cai, L., Liu, B. (2011-04-26). Phalloidin-functionalized hyperbranched conjugated polyelectrolyte for filamentous actin imaging in living Hela cells. Chemistry of Materials 23 (8) : 2113-2119. ScholarBank@NUS Repository.|
|Abstract:||A phalloidin-functionalized hyperbranched conjugated polyelectrolyte (HCPE-phalloidin) is synthesized and used for direct filamentous actin (F-actin) imaging in living Hela cells. Different from commercially available organic dye-phalloidin conjugates, which require sophisticated techniques to be delivered into living cells, simple incubation of living cells with HCPE-phalloidin leads to efficient internalization of the probe and clear visualization of F-actin due to high brightness of HCPE and good specificity between phalloidin and actin. In addition, HCPE-phalloidin possesses improved photostability as compared to that for commercially available Alexa Fluor 488-phalloidin conjugates, suggesting that the new probe is promising for long-term F-actin imaging in living cells. Further fine-tuning the fluorescent property and targeting ability of HCPE-based probes could lead to more complicated imaging applications and subcellular target detection. © 2011 American Chemical Society.|
|Source Title:||Chemistry of Materials|
|Appears in Collections:||Staff Publications|
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