Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.joen.2010.02.008
Title: Nanoparticulates for antibiofilm treatment and effect of aging on its antibacterial activity
Authors: Shrestha, A.
Zhilong, S. 
Gee, N.K. 
Kishen, A.
Keywords: Antibacterial
antibiofilm
chitosan
Enterococcus faecalis
nanoparticulates
zinc oxide
Issue Date: 2010
Source: Shrestha, A., Zhilong, S., Gee, N.K., Kishen, A. (2010). Nanoparticulates for antibiofilm treatment and effect of aging on its antibacterial activity. Journal of Endodontics 36 (6) : 1030-1035. ScholarBank@NUS Repository. https://doi.org/10.1016/j.joen.2010.02.008
Abstract: Introduction: Issues pertaining to the effective elimination of bacterial biofilm and disruption of biofilm structure still remains in endodontic disinfection. Nanoparticulates such as chitosan (CS-np) and zinc oxide (ZnO-np) are known to possess significant antibacterial properties. This study aimed to test (1) the efficacy of CS-np and ZnO-np in disinfecting and disrupting biofilm bacteria and (2) the long-term efficacy of these nanoparticulates following aging. Methods: Enterococcus faecalis (ATCC & OG1RF) in planktonic and biofilm forms were treated with different concentrations of CS-np and ZnO-np. The treated bacteria were quantified by using microbiologic methods. The biofilm viability and structure after nanoparticulate treatment were assessed by using confocal laser scanning microscopy. The effect of aging by using sterile saliva and phosphate-buffered saline on the antibacterial properties of the nanoparticulates was also determined. Results: The rate of bacterial killing by the nanoparticulates depended on the concentration and time of interaction. Total elimination of planktonic bacteria was observed in contrast to the biofilm bacteria, which survived even after 72 hours. The confocal microscopy images showed predominantly dead bacterial cells and significant reduction in the thickness of biofilm (P
Source Title: Journal of Endodontics
URI: http://scholarbank.nus.edu.sg/handle/10635/89558
ISSN: 00992399
DOI: 10.1016/j.joen.2010.02.008
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

74
checked on Feb 20, 2018

WEB OF SCIENCETM
Citations

59
checked on Feb 20, 2018

Page view(s)

26
checked on Feb 18, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.