Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2010.05.018
Title: Liquid crystals decorated with linear oligopeptide FLAG for applications in immunobiosensors
Authors: Bi, X. 
Yang, K.-L. 
Keywords: FLAG
Immunobiosensor
Liquid crystals
Oligopeptide
Issue Date: Sep-2010
Citation: Bi, X., Yang, K.-L. (2010-09). Liquid crystals decorated with linear oligopeptide FLAG for applications in immunobiosensors. Biosensors and Bioelectronics 26 (1) : 107-111. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2010.05.018
Abstract: Immunobiosensors are emerging as powerful tools for diagnostic applications. In this paper, we exploit optical properties of liquid crystal (LC) and the specific epitope-antibody interaction to develop an optical immunobiosensor which can be used to detect monoclonal anti-FLAG M2, a model antibody. The sensitive layer of this immunobiosensor is a monolayer of immobilized oligopeptide CDYKDDDDK (FLAG) at an LC/aqueous interface. The linear oligopeptide can function as an epitope which can be recognized by anti-FLAG M2. When the LC is modified with 50 μM of FLAG and then exposed to an aqueous solution containing anti-FLAG, anti-FLAG binds to the immobilized FLAG epitope and triggers orientational transition of LC. Because of the optical birefringence of the LC, the orientational transition is accompanied by changes in the optical images of LC from dark to bright under crossed polarizers. The detection limit for the system is 27. ng/mL of anti-FLAG, and the response time is
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/89339
ISSN: 09565663
DOI: 10.1016/j.bios.2010.05.018
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