Please use this identifier to cite or link to this item: https://doi.org/10.1007/s10856-013-4868-7
Title: In vitro endothelialization of cobalt chromium alloys with micro/nanostructures using adipose-derived stem cells
Authors: Shi, Z. 
Neoh, K.G. 
Kang, E.T. 
Issue Date: Apr-2013
Citation: Shi, Z., Neoh, K.G., Kang, E.T. (2013-04). In vitro endothelialization of cobalt chromium alloys with micro/nanostructures using adipose-derived stem cells. Journal of Materials Science: Materials in Medicine 24 (4) : 1067-1077. ScholarBank@NUS Repository. https://doi.org/10.1007/s10856-013-4868-7
Abstract: In this study, integrin expression, proliferation, and endothelial differentiation of adipose-derived stem cells (ADSCs) on pristine cobalt chrome (CoCr) surface, microstructured and nanostructured CoCr surfaces (obtained after treatment with piranha solution) were investigated. The results showed that proliferation of ADSCs on the substrates treated with piranha solution is not significantly different from that on the pristine substrates. However, quantitative real-time PCR analysis showed significantly enhanced up-regulation of CD31, vWF and eNOS from gene level by ADSCs on the nanostructured substrates but not on the microstructured substrates. The adsorption of vitronectin from the culture medium on the nanostructured substrates was higher than on the pristine and microstructured substrates. We speculate that this results in increased integrin αvβ3 expression in the ADSCs, which may contribute partially to the enhanced endothelial differentiation of ADSCs on the nanostructured substrates. This study shows that ADSCs can be used to endothelialize stents in vitro and the endothelial differentiation of ADSC is enhanced on the nanostructured surfaces. © 2013 Springer Science+Business Media New York.
Source Title: Journal of Materials Science: Materials in Medicine
URI: http://scholarbank.nus.edu.sg/handle/10635/89216
ISSN: 09574530
DOI: 10.1007/s10856-013-4868-7
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

7
checked on Jul 14, 2018

WEB OF SCIENCETM
Citations

5
checked on Jun 19, 2018

Page view(s)

36
checked on May 25, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.