Please use this identifier to cite or link to this item:
|Title:||In vitro and in vivo evaluation of methoxy polyethylene glycol-polylactide (MPEG-PLA) nanoparticles for small-molecule drug chemotherapy|
|Authors:||Dong, Y. |
Oral drug delivery
|Source:||Dong, Y., Feng, S.-S. (2007-10). In vitro and in vivo evaluation of methoxy polyethylene glycol-polylactide (MPEG-PLA) nanoparticles for small-molecule drug chemotherapy. Biomaterials 28 (28) : 4154-4160. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2007.05.026|
|Abstract:||Methoxy polyethylene glycol-polylactide (MPEG-PLA) nanoparticles (NPs) were prepared by the nanoprecipitation method with particle size of 140±21 nm in diameter and drug encapsulation efficiency of 87.6±3.1%. In vitro cytotoxicity of the drug formulated in the NPs was investigated with MCF-7 cancer cells in close comparison with that of Taxol®. The in vitro cytotoxicity with MCF-7 cells showed that the NP formulation could be 33.3, 10.7, 7.7 times more effective than Taxol® after 24, 48, 72 h culture at the same drug concentration of 1 μg/ml. Confocal laser scanning microscopy (CLSM) visualized cellular internalization of the coumarin 6-loaded MPEG-PLA NPs. The in vitro results were further confirmed by the in vivo pharmacokinetic analysis with SD rats. The total area-under-the-curve (AUC0-∞), which determines the therapeutic effects of a dose, was found to be 29,600±1690 ng-h/ml for the NP formulation, which is 3.09 times of 9570±1480 ng-h/l for Taxol® with 10 mg/kg dose i.v. injection. The half-life (t1/2) of the drug formulated in the NPs was found to be 18.80±3.14 h, which is 2.75 times of 6.84±1.39 h for Taxol®. The distribution volume at steady state for the drug loaded in the NPs was 7.21±2.17 l/kg, which was 2.93 times of 2.46±1.41 l/kg for Taxol®. Our proof-of-concept in vitro and in vivo valuation shows that our MPEG-PLA NP formulation could have great advantages versus the original drug in small-molecule drug chemotherapy as well as in various applications in nanomedicine. © 2007 Elsevier Ltd. All rights reserved.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 14, 2018
WEB OF SCIENCETM
checked on Jan 16, 2018
checked on Feb 18, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.