Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bios.2008.06.054
DC Field | Value | |
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dc.title | Highly sensitive oligonucleotide-based fluorometric detection of mercury(II) in aqueous media | |
dc.contributor.author | Liu, B. | |
dc.date.accessioned | 2014-10-09T06:50:06Z | |
dc.date.available | 2014-10-09T06:50:06Z | |
dc.date.issued | 2008-12-01 | |
dc.identifier.citation | Liu, B. (2008-12-01). Highly sensitive oligonucleotide-based fluorometric detection of mercury(II) in aqueous media. Biosensors and Bioelectronics 24 (4) : 756-760. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2008.06.054 | |
dc.identifier.issn | 09565663 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/89113 | |
dc.description.abstract | This paper describes a highly sensitive and selective Hg2+ sensor using a label free Hg2+ specific probe (5′-18T-3′) and an intercalation dye SYBR Green I (SG). The Hg2+ specific probe is composed of thymines (T) and readily forms T-Hg2+-T complexes in the presence of Hg2+. This specific T-Hg2+-T formation affects the hybridization of the Hg2+ specific probe and the intercalation of SG. Upon treatment of 1 nM 5′-18T-3′ with different amount of Hg2+ (0.1-10 nM), which is followed by hybridization with 1 nM 5′-18T-3′ and incubation with 1 μL of SG, the solution fluorescence gave a linear response (R = 0.996) to the concentration of Hg2+. The detection limit for Hg2+ was 0.5 nM (0.1 ppb). The overall test only takes few minutes and very little interference is observed from non-specific metal ions. This approach may find potential applications in monitoring the Hg2+ concentration in drinking water. © 2008 Elsevier B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bios.2008.06.054 | |
dc.source | Scopus | |
dc.subject | Hg2+ sensor | |
dc.subject | Hg2+ specific probe | |
dc.subject | Intercalation | |
dc.subject | SYBR Green I | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1016/j.bios.2008.06.054 | |
dc.description.sourcetitle | Biosensors and Bioelectronics | |
dc.description.volume | 24 | |
dc.description.issue | 4 | |
dc.description.page | 756-760 | |
dc.description.coden | BBIOE | |
dc.identifier.isiut | 000261262000039 | |
Appears in Collections: | Staff Publications |
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