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Title: Highly emissive PEG-encapsulated conjugated polymer nanoparticles
Authors: Li, Y.
Liu, J.
Liu, B. 
Tomczak, N.
Issue Date: 21-Sep-2012
Citation: Li, Y., Liu, J., Liu, B., Tomczak, N. (2012-09-21). Highly emissive PEG-encapsulated conjugated polymer nanoparticles. Nanoscale 4 (18) : 5694-5702. ScholarBank@NUS Repository.
Abstract: A novel bioimaging probe based on a conjugated polymer, poly(9,9-dihexylfluorene-alt-2,1,3-benzoxadiazole) (PFBD), is demonstrated. Transfer of the hydrophobic polymer into water using a short chain poly(ethylene glycol) (PEG) resulted in conjugated polymer nanoparticles (PEG-PFBD) with a fluorescence quantum yield of 46%. The PEG-PFBD nanoparticles possessed several desirable structural and photophysical properties, such as colloidal stability in a broad range of pH values, sub-20 nm particle size, the presence of surface chemical functionality, as well as desirable excitation and emission spectra, for bioimaging applications. PEG-PFBD nanoparticles were conjugated with cyclic RGDfK targeting peptide for labeling of membrane α Vβ 3 integrin receptors on live HT-29 adenocarcinoma cells. Single nanoparticle microscopy revealed that the PEG-capped PFBD nanoparticles exhibit at least ten times higher emitted photon counts than single quantum dots (QD655) of comparable size. In addition, Fluorescence Lifetime Imaging Microscopy (FLIM) of single PEG-PFBD nanoparticles revealed that the nanoparticles display a clearly resolvable single nanoparticle fluorescence lifetime. This journal is © The Royal Society of Chemistry 2012.
Source Title: Nanoscale
ISSN: 20403364
DOI: 10.1039/c2nr31267k
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