Please use this identifier to cite or link to this item:
|Title:||Highly emissive PEG-encapsulated conjugated polymer nanoparticles|
|Citation:||Li, Y., Liu, J., Liu, B., Tomczak, N. (2012-09-21). Highly emissive PEG-encapsulated conjugated polymer nanoparticles. Nanoscale 4 (18) : 5694-5702. ScholarBank@NUS Repository. https://doi.org/10.1039/c2nr31267k|
|Abstract:||A novel bioimaging probe based on a conjugated polymer, poly(9,9-dihexylfluorene-alt-2,1,3-benzoxadiazole) (PFBD), is demonstrated. Transfer of the hydrophobic polymer into water using a short chain poly(ethylene glycol) (PEG) resulted in conjugated polymer nanoparticles (PEG-PFBD) with a fluorescence quantum yield of 46%. The PEG-PFBD nanoparticles possessed several desirable structural and photophysical properties, such as colloidal stability in a broad range of pH values, sub-20 nm particle size, the presence of surface chemical functionality, as well as desirable excitation and emission spectra, for bioimaging applications. PEG-PFBD nanoparticles were conjugated with cyclic RGDfK targeting peptide for labeling of membrane α Vβ 3 integrin receptors on live HT-29 adenocarcinoma cells. Single nanoparticle microscopy revealed that the PEG-capped PFBD nanoparticles exhibit at least ten times higher emitted photon counts than single quantum dots (QD655) of comparable size. In addition, Fluorescence Lifetime Imaging Microscopy (FLIM) of single PEG-PFBD nanoparticles revealed that the nanoparticles display a clearly resolvable single nanoparticle fluorescence lifetime. This journal is © The Royal Society of Chemistry 2012.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 22, 2018
WEB OF SCIENCETM
checked on Sep 12, 2018
checked on Aug 10, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.