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https://doi.org/10.1021/am100591t
DC Field | Value | |
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dc.title | Glucose biosensor from covalent immobilization of chitosan-coupled carbon nanotubes on polyaniline-modified gold electrode | |
dc.contributor.author | Wan, D. | |
dc.contributor.author | Yuan, S. | |
dc.contributor.author | Li, G.L. | |
dc.contributor.author | Neoh, K.G. | |
dc.contributor.author | Kang, E.T. | |
dc.date.accessioned | 2014-10-09T06:48:57Z | |
dc.date.available | 2014-10-09T06:48:57Z | |
dc.date.issued | 2010-11-24 | |
dc.identifier.citation | Wan, D., Yuan, S., Li, G.L., Neoh, K.G., Kang, E.T. (2010-11-24). Glucose biosensor from covalent immobilization of chitosan-coupled carbon nanotubes on polyaniline-modified gold electrode. ACS Applied Materials and Interfaces 2 (11) : 3083-3091. ScholarBank@NUS Repository. https://doi.org/10.1021/am100591t | |
dc.identifier.issn | 19448244 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/89013 | |
dc.description.abstract | An amperometric glucose biosensor was prepared using polyaniline (PANI) and chitosan-coupled carbon nanotubes (CS-CNTs) as the signal amplifiers and glucose oxidase (GOD) as the glucose detector on a gold electrode (the Au-g-PANI-c-(CS-CNTs)-GOD biosensor). The PANI layer was prepared via oxidative graft polymerization of aniline from the gold electrode surface premodified by self-assembled monolayer of 4-aminothiophenol. CS-CNTs were covalently coupled to the PANI-modified gold substrate using glutaradehyde as a bifunctional linker. GOD was then covalently bonded to the pendant hydroxyl groups of chitosan using 1,4-carbonyldiimidazole as the bifunctional linker. The surface functionalization processes were ascertained by X-ray photoelectron spectroscopy (XPS) analyses. The field emission scanning electron microscopy (FESEM) images of the Au-g-PANI-c-(CS-CNTs) electrode revealed the formation of a three-dimensional surface network structure. The electrode could thus provide a more spatially biocompatible microenvironment to enhance the amount and biocatalytic activity of the immobilized enzyme and to better mediate the electron transfer. The resulting Au-g-PANI-c-(CS-CNTs)-GOD biosensor exhibited a linear response to glucose in the concentration range of 1-20 mM, good sensitivity (21 μA/(mM•cm2)), good reproducibility, and retention of >80% of the initial response current after 2 months of storage. © 2010 American Chemical Society. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/am100591t | |
dc.source | Scopus | |
dc.subject | Au electrode | |
dc.subject | carbon nanotubes | |
dc.subject | chitosan | |
dc.subject | glucose biosensor | |
dc.subject | polyaniline | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1021/am100591t | |
dc.description.sourcetitle | ACS Applied Materials and Interfaces | |
dc.description.volume | 2 | |
dc.description.issue | 11 | |
dc.description.page | 3083-3091 | |
dc.identifier.isiut | 000284454400019 | |
Appears in Collections: | Staff Publications |
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