Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.colsurfb.2011.07.036
Title: Formulation design, preparation and physicochemical characterizations of solid lipid nanoparticles containing a hydrophobic drug: Effects of process variables
Authors: Das, S.
Ng, W.K.
Kanaujia, P.
Kim, S.
Tan, R.B.H. 
Keywords: Drug release
Encapsulation
Hydrophobic drug
Solid lipid nanoparticle
Tretinoin
Issue Date: 1-Nov-2011
Source: Das, S., Ng, W.K., Kanaujia, P., Kim, S., Tan, R.B.H. (2011-11-01). Formulation design, preparation and physicochemical characterizations of solid lipid nanoparticles containing a hydrophobic drug: Effects of process variables. Colloids and Surfaces B: Biointerfaces 88 (1) : 483-489. ScholarBank@NUS Repository. https://doi.org/10.1016/j.colsurfb.2011.07.036
Abstract: This study aimed to prepare solid lipid nanoparticles (SLNs) of a hydrophobic drug, tretinoin, by emulsification-ultrasonication method. Solubility of tretinoin in the solid lipids was examined. Effects of process variables were investigated on particle size, polydispersity index (PI), zeta potential (ZP), drug encapsulation efficiency (EE), and drug loading (L) of the SLNs. Shape and surface morphology of the SLNs were investigated by cryogenic field emission scanning electron microscopy (cryo-FESEM). Complete encapsulation of drug in the nanoparticles was checked by cross-polarized light microscopy and differential scanning calorimetry (DSC). Crystallinity of the formulation was analyzed by DSC and powder X-ray diffraction (PXRD). In addition, drug release and stability studies were also performed. The results indicated that 10mg tretinoin was soluble in 0.45±0.07g Precirol ® ATO5 and 0.36±0.06g Compritol ® 888ATO, respectively. Process variables exhibited significant influence in producing SLNs. SLNs with 75% EE, and ∼0.8% L can be produced following the appropriate formulation conditions. Cryo-FESEM study showed spherical particles with smooth surface. Cross-polarized light microscopy study revealed that drug crystals in the external aqueous phase were absent when the SLNs were prepared at ≤0.05% drug concentration. DSC and PXRD studies indicated complete drug encapsulation within the nanoparticle matrix as amorphous form. The drug release study demonstrated sustained/prolonged drug release from the SLNs. Furthermore, tretinoin-loaded SLNs were stable for 3 months at 4°C. Hence, the developed SLNs can be used as drug carrier for sustained/prolonged drug release and/or to improve oral absorption/bioavailability. © 2011 Elsevier B.V.
Source Title: Colloids and Surfaces B: Biointerfaces
URI: http://scholarbank.nus.edu.sg/handle/10635/88955
ISSN: 09277765
DOI: 10.1016/j.colsurfb.2011.07.036
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

86
checked on Feb 19, 2018

WEB OF SCIENCETM
Citations

74
checked on Feb 12, 2018

Page view(s)

29
checked on Feb 20, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.