Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.enzmictec.2009.06.001
Title: Fast biodegradation of long chain n-alkanes and crude oil at high concentrations with Rhodococcus sp. Moj-3449
Authors: Binazadeh, M.
Karimi, I.A. 
Li, Z. 
Keywords: Biodegradation
Bioremediation
Long-chain n-alkanes
n-Hexadecane
Oil degradation
Subterminal oxidation
Issue Date: 7-Sep-2009
Citation: Binazadeh, M., Karimi, I.A., Li, Z. (2009-09-07). Fast biodegradation of long chain n-alkanes and crude oil at high concentrations with Rhodococcus sp. Moj-3449. Enzyme and Microbial Technology 45 (3) : 195-202. ScholarBank@NUS Repository. https://doi.org/10.1016/j.enzmictec.2009.06.001
Abstract: Biodegradation of long chain n-alkanes and crude oil with fast rate and high concentration are desirable for bioremediation, especially in heavily oil-polluted areas, and enhanced oil recovery. We discovered Rhodococcus sp. Moj-3449 with such unique abilities by screening microorganisms for the growth on n-hexadecane at 30 mg/mL. The new strain grew very fast on 120 mg/mL of n-hexadecane giving a cell density of 14.7 g cdw/L after only 2 days' incubation. During the growth with this strain, the oil-water phases were rapidly emulsified, giving rise to tolerance to high alkane concentration (250 mg/mL) and fast growth rate of 0.10-0.20 h-1 for alkane concentration of 1-180 mg/mL. The degraded concentration of n-hexadecane increased linearly with the initial alkane concentration (1-250 mg/mL). Incubation on n-hexadecane at 250 mg/mL for 7 days gave a cell density of 13.5 g cdw/L and degraded 124 mg/mL of n-hexadecane. The strain grew also fast on n-dodecane (C12), n-tetradecane (C14), and n-octadecane (C18), with degradation preference of C14 (=C16) > C12 > C18. Different from many alkane-degrading strains, Rhodococcus sp. Moj-3449 was found to have subterminal oxidation pathway. Rhodococcus sp. Moj-3449 degraded also crude oil fast at 60-250 mg/mL, with a wide range of n-alkanes (C10-C35) as substrates in which C14-C19 are preferred. The degradation ability increased with initial oil concentration from 60 to 150 mg/mL and slightly decreased afterwards. Incubation on 150 mg/mL of crude oil for 7 days degraded 37% of n-alkanes. The outstanding ability of rapidly degrading long chain n-alkanes and crude oil at high concentration makes Rhodococcus sp. Moj-3449 potentially useful for bioremediation and microbial enhanced oil recovery. © 2009 Elsevier Inc. All rights reserved.
Source Title: Enzyme and Microbial Technology
URI: http://scholarbank.nus.edu.sg/handle/10635/88903
ISSN: 01410229
DOI: 10.1016/j.enzmictec.2009.06.001
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

44
checked on Jul 14, 2018

WEB OF SCIENCETM
Citations

40
checked on Jun 18, 2018

Page view(s)

55
checked on May 25, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.