Please use this identifier to cite or link to this item:
|Title:||Enhanced endothelial differentiation of adipose-derived stem cells by substrate nanotopography|
|Authors:||Shi, Z. |
|Keywords:||Adipose-derived stem cells|
|Citation:||Shi, Z., Neoh, K.G., Kang, E.T., Poh, C.K., Wang, W. (2014-01). Enhanced endothelial differentiation of adipose-derived stem cells by substrate nanotopography. Journal of Tissue Engineering and Regenerative Medicine 8 (1) : 50-58. ScholarBank@NUS Repository. https://doi.org/10.1002/term.1496|
|Abstract:||Adipose-derived stem cells (ADSCs) have great potential as a cell source for tissue engineering and regenerative medicine because they are easier to obtain, have lower donor-site morbidity and are available in larger numbers than stem cells harvested using bone marrow aspiration. Until now, little has been known about how nanotopography affects the proliferation and endothelial differentiation of ADSCs. In the present study, two nanograting substrates with a period (ridge and groove) of about 250 and 500 nm, respectively, were fabricated on quartz and their effect on ADSC fate was investigated. The results showed that proliferation of ADSCs on nanograting substrates decreased while cell attachment was not significantly affected compared to a flat substrate. Endothelial differentiation of ADSCs on both flat and nanograting substrates can be induced with vascular endothelial growth factor, as shown by immunofluorescent staining. Quantitative real-time PCR analysis showed significantly enhanced upregulation of vWF, PECAM-1 and VE-cadherin at the gene level by ADSCs on the nanograting substrates. In vitro angiogenesis assay on Matrigel showed that nanograting substrates enhanced capillary tube formation. This study highlights the beneficial influence of nanotopography on the differentiation of ADSC into endothelial cells which play an important role in vascularization. © 2012 John Wiley & Sons, Ltd.|
|Source Title:||Journal of Tissue Engineering and Regenerative Medicine|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 21, 2019
WEB OF SCIENCETM
checked on Mar 12, 2019
checked on Feb 8, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.