Please use this identifier to cite or link to this item:
Title: DNA microarrays on ultraviolet-modified surfaces for speciation of bacteria
Authors: Liu, Y.
He, J. 
Yang, K.-L. 
Keywords: DNA microarray
Surface modification
Issue Date: 15-Feb-2014
Citation: Liu, Y., He, J., Yang, K.-L. (2014-02-15). DNA microarrays on ultraviolet-modified surfaces for speciation of bacteria. Analytical Biochemistry 447 (1) : 156-161. ScholarBank@NUS Repository.
Abstract: In this study, we report an approach to activate inert hydrocarbon monolayers with ultraviolet (UV) light to fabricate DNA microarrays. Unlike traditional microarrays that require reactive functional groups on the surface, our DNA microarray is built on an inert layer of N,N-dimethyl-N-octadecyl(3- aminopropyl)trimethoxysilyl chloride silane (DMOAP). This layer is activated by UV (254 nm) just prior to the immobilization of oligonucleotide probes. Our X-ray photoelectron spectroscopy (XPS) results show that new functional groups such as alcohol (C-O), aldehyde (CO), and carboxylic acid (OCO) form on the surface after the UV exposure. Among them, aldehyde groups are responsible for the immobilization of amine-label oligonucleotides. By using this approach, we further optimize UV exposure time and oligonucleotide concentration and also reduce agent concentration to achieve a high density of immobilized oligonucleotides up to 0.16 pmol/mm2. As a proof of concept, we demonstrate that this microarray can be used for differentiation of different Clostridium species such as Clostridium acetobutylicum, Clostridium butylicum, and Clostridium beijerinkii. © 2013 Elsevier B.V. All rights reserved.
Source Title: Analytical Biochemistry
ISSN: 00032697
DOI: 10.1016/j.ab.2013.10.033
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jan 16, 2019


checked on Jan 16, 2019

Page view(s)

checked on Jan 12, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.