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Title: Conjugated polyelectrolytes as light-up macromolecular probes for heparin sensing
Authors: Pu, K.-Y. 
Liu, B. 
Issue Date: 23-Jan-2009
Citation: Pu, K.-Y., Liu, B. (2009-01-23). Conjugated polyelectrolytes as light-up macromolecular probes for heparin sensing. Advanced Functional Materials 19 (2) : 277-284. ScholarBank@NUS Repository.
Abstract: Two cationic poly(fluorene-alt-benzothiadiazole)s with different side chains are designed and synthesized. Both polymers show low fluorescence in aqueous solution due to the charge-transfer character of the polymer's excited states. Fluorescence turn-on biosensors for heparin detection and quantification are developed, taking advantage of complexation-induced aggregation, which increases the polymer fluorescence in aqueous solution. It is found that good polymer water-solubility is beneficial to the sensitivity and fluorescence contrast of the heparin turn-on sensor as a result of the low fluorescence background. Moreover, stronger complexation between the polymer/heparin leads to a substantially larger fluorescence increase in the presence of heparin relative to that in the presence of its analog, hyaluronic acid (HA), allowing discrimination of heparin from HA. Heparin quantification with a practical calibration range covering the whole therapeutic dosing levels (0.2-8 U mL -1) is realized based on the polymer with good water-solubility. This investigation provides a new insight for designing conjugated polymers with a light-up signature for biomolecular sensing. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA.
Source Title: Advanced Functional Materials
ISSN: 1616301X
DOI: 10.1002/adfm.200800960
Appears in Collections:Staff Publications

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