Please use this identifier to cite or link to this item: https://doi.org/10.1002/bit.21824
Title: Characterization of porous poly(D,L-Lactic-co-glycolic Acid) sponges fabricated by supercritical CO2 gas-foaming method as a scaffold for three-dimensional growth of hep3b cells
Authors: Zhu, X.H. 
Lee, L.Y. 
Jackson, J.S.H.
Tong, Y.W. 
Wang, C.-H. 
Keywords: Liver tissue engineering
PLGA
Sponge
Supercritical CO2 foaming
Issue Date: 1-Aug-2008
Citation: Zhu, X.H., Lee, L.Y., Jackson, J.S.H., Tong, Y.W., Wang, C.-H. (2008-08-01). Characterization of porous poly(D,L-Lactic-co-glycolic Acid) sponges fabricated by supercritical CO2 gas-foaming method as a scaffold for three-dimensional growth of hep3b cells. Biotechnology and Bioengineering 100 (5) : 998-1009. ScholarBank@NUS Repository. https://doi.org/10.1002/bit.21824
Abstract: This study presents the application of the porous poly(D,L-lactic-co- glycolic acid) (PLGA) sponges fabricated from an organic solvent free supercritical gas foaming technique. Two formulations of PLGA sponges with different co-polymer compositions (85:15 and 50:50) were fabricated as novel scaffolds to guide human hepatoma cell line, Hep3B cell growth in vitro. The PLGA sponges showed desirable biodegradability and exhibited uniform pore size distribution with moderate interconnectivity. It was observed in this study that cells cultured on PLGA sponges showed lower proliferation rate as compared to the control during 14 days of culture as measured by using total DNA and methylthiazol tetrazolium (MTT) assays. However, the cells cultured on the sponges tended to aggregate to form cell islets which were able to express better hepatic functions. The enzyme-linked immunosorbent assay (ELISA) results showed that the cell-sponge constructs secreted 1.5-3.0 times more albumin than the control when normalized to cellular content. In a similar fashion, its detoxification ability was also predominantly higher than that of the control as indicated by the ethoxyresorufm-O-deethylase (EROD) results. By comparing the cells growing on the two formulations of PLGA sponges, it was found that the PLGA 85:15 sponge exhibited better conductive and desirable environment for hep3B cells as justified by better cell infiltration, higher proliferation and hepatic function than the PLGA 50:50 sponge. © 2008 Wiley Periodicals, Inc.
Source Title: Biotechnology and Bioengineering
URI: http://scholarbank.nus.edu.sg/handle/10635/88639
ISSN: 00063592
DOI: 10.1002/bit.21824
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

52
checked on Sep 19, 2018

WEB OF SCIENCETM
Citations

42
checked on Sep 19, 2018

Page view(s)

54
checked on Sep 7, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.